Simultaneous enrichment and separation of flavonoids from Herba Epimedii by macroporous resins coupled with preparative chromatographic method

被引：11

作者：

Zhou, Zhengzheng ^{[1
]}

Luo, Jianguang ^{[1
]}

Wang, Junsong ^{[1
]}

Li, Liang ^{[1
]}

Kong, Lingyi ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Jiangsu, Peoples R China

来源：

NATURAL PRODUCT RESEARCH | 2015年 / 29卷 / 02期

基金：

中国国家自然科学基金;

关键词：

epimedin C; icariin; epimedin B; separation; Epimedium; epimedin A; COUNTER-CURRENT CHROMATOGRAPHY; PURIFICATION; EXTRACTS; ICARIIN;

D O I：

10.1080/14786419.2014.964704

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An efficient, feasible enrichment and separation method of epimedins A, B, C and icariin from Herba Epimedii was developed by the combination of microwave-assisted extraction, macroporous resins and preparative HPLC. WDX-5 macroporous resin shows better recoveries at 96.2%, 97.0%, 98.2% and 97.1% for epimedins A, B, C and icariin than other macroporous resins used in the experiments. As a result, epimedins A (5.1mg), B (15.3mg), C (7.6mg) and icariin (14.3mg) were obtained from 6.0g crude Herba Epimedii with the recoveries at 70.8%, 68.9%, 66.7% and 95.3%, respectively. The method developed in this study may provide scientific references for the enrichment and separation of flavonoids from Herba Epimedii.

引用

页码：185 / 188

页数：4

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