Label-Free Imaging of Single Neuron Activities by Stimulated Raman Scattering

被引:0
|
作者
Zhang, Delong [1 ]
Lee, Hyeon Jeong [2 ]
Shih, Pei-Yu [3 ]
Drenan, Ryan E. [3 ]
Cheng, Ji-Xin [1 ]
机构
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47906 USA
[2] Purdue Univ, Dept Comparat Pathobiol, W Lafayette, IN 47906 USA
[3] Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47906 USA
关键词
D O I
暂无
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
We report the observation of single neuron activities by spectral focusing stimulated Raman scattering (SRS) microscopy. Changes in SRS signal intensity in live neurons correlate to action potentials indicated by fluorescent calcium indicator.
引用
收藏
页数:2
相关论文
共 50 条
  • [41] Initial experience with label-free stimulated Raman scattering microscopy for intraoperative assessment of peripheral nerves
    Wilson, Thomas J.
    Toland, Angus
    Cayrol, Romain
    Vogel, Hannes
    CLINICAL NEUROLOGY AND NEUROSURGERY, 2022, 214
  • [42] Label-Free Raman Hyperspectral Imaging of Single Cells Cultured on Polymer Substrates
    Sinjab, Faris
    Sicilia, Giovanna
    Shipp, Dustin W.
    Marlow, Maria
    Notingher, Ioan
    APPLIED SPECTROSCOPY, 2017, 71 (12) : 2595 - 2607
  • [43] Label-free histomorphometry of peripheral nerve by stimulated Raman spectroscopy
    Coto Hernandez, Ivan
    Yang, Wenlong
    Mohan, Suresh
    Jowett, Nate
    MUSCLE & NERVE, 2020, 62 (01) : 137 - 142
  • [44] LABEL-FREE COHERENT RAMAN SCATTERING (CRS) IMAGING FOR METABOLIC PROFILING OF NEMATODES.
    Holden-Dye, L.
    Dalliere, N.
    Urwin, P.
    Lilly, C.
    O'Connor, V.
    Pertek, J.
    Mahajan, S.
    JOURNAL OF NEMATOLOGY, 2014, 46 (02) : 175 - 176
  • [45] Integrated coherent Raman scattering and multiphoton microscopy for label-free imaging of dentin in the tooth
    Wang, Zi
    Zheng, Wei
    Lin, Jian
    Hsu, Chin-Ying
    Huang, Zhiwei
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIV, 2014, 8948
  • [46] Enhancing the sensitivity and robustness of label-free imaging systems via stimulated Raman adiabatic passage
    Leon-Montiel, R. de J.
    Torres, Juan P.
    NEW JOURNAL OF PHYSICS, 2012, 14
  • [47] Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy
    Ferrara, Maria Antonietta
    Filograna, Angela
    Ranjan, Rajeev
    Corda, Daniela
    Valente, Carmen
    Sirleto, Luigi
    PLOS ONE, 2019, 14 (05):
  • [48] Surface-Enhanced Raman Scattering for Label-Free Living Single Cell Analysis
    Kuku, Gamze
    Altunbek, Mine
    Culha, Mustafa
    ANALYTICAL CHEMISTRY, 2017, 89 (21) : 11160 - 11166
  • [49] Label-free Imaging of Human Heart by Raman Microspectroscopy
    Yamamoto, Tsunehisa
    Harada, Yoshinori
    Minamikawa, Takeo
    Yamaoka, Sadahisa
    Yaku, Hitoshi
    Takamatsu, Tetsuro
    CIRCULATION, 2013, 128 (22)
  • [50] Label-free biological imaging by CARS/Raman microscopy
    Fujita, Katsumasa
    NEUROSCIENCE RESEARCH, 2009, 65 : S3 - S3