Look and See if It Is Time To Induce Protein Expression in Escherichia coli Cultures

被引：7
|
作者：
Kelley, K. Danielle
Olive, Lorenzo Q.
Hadziselimovic, Anna
Sanders, Charles R. [1 ]
机构：
[1] Vanderbilt Univ, Dept Biochem, Sch Med, Nashville, TN 37232 USA
来源：
BIOCHEMISTRY | 2010年 / 49卷 / 26期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1021/bi1007194
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It is shown that Methyl Red can be used as an indicator dye that changes color in Escherichia coli culture as a result of time- and cell density-dependent bleaching by azoreductase produced by the bacteria. For cell cultures that are being used to express a recombinant protein, this phenomenon can be exploited to provide a simple visual cue that cell cultures have reached an appropriate growth phase for addition of an agent to induce protein expression, such as isopropyl thiogalactoside.
引用
收藏
页码：5405 / 5407
页数：3
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