Look and See if It Is Time To Induce Protein Expression in Escherichia coli Cultures

被引：7
|
作者：
Kelley, K. Danielle
Olive, Lorenzo Q.
Hadziselimovic, Anna
Sanders, Charles R. [1 ]
机构：
[1] Vanderbilt Univ, Dept Biochem, Sch Med, Nashville, TN 37232 USA
来源：
BIOCHEMISTRY | 2010年 / 49卷 / 26期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1021/bi1007194
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It is shown that Methyl Red can be used as an indicator dye that changes color in Escherichia coli culture as a result of time- and cell density-dependent bleaching by azoreductase produced by the bacteria. For cell cultures that are being used to express a recombinant protein, this phenomenon can be exploited to provide a simple visual cue that cell cultures have reached an appropriate growth phase for addition of an agent to induce protein expression, such as isopropyl thiogalactoside.
引用
收藏
页码：5405 / 5407
页数：3
相关论文
共 50 条
  • [31] Does UVB radiation induce SoxS gene expression in Escherichia coli cells?
    A. A. Gomes
    L. M. B. O. Asad
    I. Felzenszwalb
    A. C. Leitão
    A. B. Silva
    H. C. R. Guillobel
    N. R. Asad
    Radiation and Environmental Biophysics, 2004, 43 : 219 - 222
  • [32] Soft-metal(loid)s induce protein aggregation in Escherichia coli
    Cornejo, Fabian A.
    Munoz-Villagran, Claudia
    Luraschi, Roberto A.
    Sandoval-Diaz, Maria P.
    Cancino, Camila A.
    Pugin, Benoit
    Morales, Eduardo H.
    Piotrowski, Jeff S.
    Sandoval, Juan M.
    Vasquez, Claudio C.
    Arenas, Felipe A.
    FRONTIERS IN MICROBIOLOGY, 2023, 14
  • [33] Reduction of acetate accumulation in Escherichia coli cultures for increased recombinant protein production
    Wong, Matthew S.
    Wu, Steven
    Causey, Thomas B.
    Bennett, George N.
    San, Ka-Yiu
    METABOLIC ENGINEERING, 2008, 10 (02) : 97 - 108
  • [34] Protein disorder is positively correlated with gene expression in Escherichia coli
    Paliy, Oleg
    Gargac, Shawn M.
    Cheng, Yugong
    Uversky, Vladimir N.
    Dunker, A. Keith
    JOURNAL OF PROTEOME RESEARCH, 2008, 7 (06) : 2234 - 2245
  • [35] EXPRESSION OF A SYNTHETIC MUSSEL ADHESIVE PROTEIN IN ESCHERICHIA-COLI
    SALERNO, AJ
    GOLDBERG, I
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1992, 203 : 201 - PMSE
  • [36] EXPRESSION OF A PROLINE-ENRICHED PROTEIN IN ESCHERICHIA-COLI
    KANGAS, TT
    COONEY, CL
    GOMEZ, RF
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 43 (03) : 629 - 635
  • [37] Construction of pMH, a convenient Escherichia coli protein expression vector
    Gortchakov, AA
    Kaltenhäuser, J
    Eggert, H
    Saumweber, H
    MOLECULAR BIOLOGY, 2004, 38 (04) : 600 - 602
  • [38] Expression in Escherichia coli and simple purification of human Fhit protein
    Pawelczyk, T
    Kowara, R
    Golebiowski, F
    Matecki, A
    PROTEIN EXPRESSION AND PURIFICATION, 2000, 18 (03) : 320 - 326
  • [39] DnaA protein stimulates polA gene expression in Escherichia coli
    Quinones, A
    Wandt, G
    Kleinsteuber, S
    Messer, W
    MOLECULAR MICROBIOLOGY, 1997, 23 (06) : 1193 - 1202
  • [40] EXPRESSION OF RIBOSOMAL-PROTEIN GENES IN ESCHERICHIA-COLI
    CABEZON, T
    BOLLEN, A
    FAELEN, M
    ARCHIVES INTERNATIONALES DE PHYSIOLOGIE DE BIOCHIMIE ET DE BIOPHYSIQUE, 1974, 82 (01): : 172 - 172
12345