Isolation and characterization of polymorphic microsatellite markers for the endangered Korean freshwater fish Hemibarbus mylodon

被引：1
|
作者：
Kim, Woo-Jin [1 ]
Lee, Yoon-A
Bang, In-Chul
机构：
[1] Natl Fisheries Res & Dev Inst, Biotechnol Res Ctr, Pusan 619902, South Korea
[2] Soonchunhyang Univ, Dept Marine Biotechnol, Asan 336745, South Korea
来源：
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 03期
关键词：
endangered species; genetic diversity; Hemibarbus mylodon; Korean barbell; microsatellite markers;
D O I：
10.1111/j.1471-8286.2006.01639.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Korean barbel, Hemibarbus mylodon, is an endangered freshwater species endemic to Korea. In order to undertake a conservation program for this species in Korea, it is essential to evaluate its genetic diversity and population structure. For this purpose, we developed 10 polymorphic microsatellite markers and examined their allelic variation using a total of 60 wild individuals collected from three different localities. The number of alleles per locus ranged from three to 26. The expected and observed heterozygosities per locus ranged from 0.19 to 0.94 and from 0.20 to 0.98, respectively. Significant departures from Hardy-Weinberg equilibrium expectations occurred in four loci. These markers will be useful for the assessment of genetic diversity for this species.
引用
收藏
页码：516 / 518
页数：3
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