A cytoplasmic AAA family peroxin, Pex1p, interacts with Pex6p

被引：56

作者：

Tamura, S ^{[1
]}

Shimozawa, N

Suzuki, Y

Tsukamoto, T

Osumi, T

Fujiki, Y

机构：

[1] Kyushu Univ, Fac Sci, Dept Biol, Fukuoka 8128581, Japan

[2] Gifu Univ, Sch Med, Dept Pediat, Gifu 5008076, Japan

[3] Himeji Inst Technol, Dept Life Sci, Kamigori, Hyogo 6781297, Japan

[4] Sci & Technol Corp Japan, CREST, Tokyo 1700001, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 245卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.8522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human PEX1 (HsPEX1) is the causative gene for peroxisome-deficiency disorders such as Zellweger syndrome of complementation group I, encoding the peroxin, Pex1p, a member of AAA family. Pex1p tagged with an epitope flag was expressed in wild-type Chinese hamster ovary (CHO) cell, CHO-K1. Pex1p was localized in the cytoplasm, as assessed by immunofluorescent microscopy. Cell-lysate of HsPEX1-transfected CHO-K1 was incubated with in vitro synthesized S-35-labelled Pex6p, an AAA family peroxin. Immunoprecipitation of Pex1p using anti-Pex1p antibody resulted in concomitant recovery of S-35-Pex6p. Conversely, S-35-Pex1p was obtained in immunoprecipitate from CHO-K1 expressing human Pex6p, using anti-Pex6p antibody. These results strongly suggest that Pex1p and Pex6p interact with each other, (C) 1998 Academic Press.

引用

页码：883 / 886

页数：4

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