A cytoplasmic AAA family peroxin, Pex1p, interacts with Pex6p

被引：56
|
作者：
Tamura, S [1 ]
Shimozawa, N
Suzuki, Y
Tsukamoto, T
Osumi, T
Fujiki, Y
机构：
[1] Kyushu Univ, Fac Sci, Dept Biol, Fukuoka 8128581, Japan
[2] Gifu Univ, Sch Med, Dept Pediat, Gifu 5008076, Japan
[3] Himeji Inst Technol, Dept Life Sci, Kamigori, Hyogo 6781297, Japan
[4] Sci & Technol Corp Japan, CREST, Tokyo 1700001, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 245卷 / 03期
关键词：
D O I：
10.1006/bbrc.1998.8522
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human PEX1 (HsPEX1) is the causative gene for peroxisome-deficiency disorders such as Zellweger syndrome of complementation group I, encoding the peroxin, Pex1p, a member of AAA family. Pex1p tagged with an epitope flag was expressed in wild-type Chinese hamster ovary (CHO) cell, CHO-K1. Pex1p was localized in the cytoplasm, as assessed by immunofluorescent microscopy. Cell-lysate of HsPEX1-transfected CHO-K1 was incubated with in vitro synthesized S-35-labelled Pex6p, an AAA family peroxin. Immunoprecipitation of Pex1p using anti-Pex1p antibody resulted in concomitant recovery of S-35-Pex6p. Conversely, S-35-Pex1p was obtained in immunoprecipitate from CHO-K1 expressing human Pex6p, using anti-Pex6p antibody. These results strongly suggest that Pex1p and Pex6p interact with each other, (C) 1998 Academic Press.
引用
收藏
页码：883 / 886
页数：4
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