Synthesis of TMG-capped RNA-DNA chimeric oligonucleotides

被引：5

作者：

Sekine, M ^{[1
]}

Ushioda, M ^{[1
]}

Wada, T ^{[1
]}

Seio, K ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Life Sci, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

TETRAHEDRON LETTERS | 2003年 / 44卷 / 08期

关键词：

D O I：

10.1016/S0040-4039(02)02828-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

This paper deals with the synthesis of m(3)(2,2.7)G(5')pppAmpUmpApd(CpTpTpApCpCpT), a 5'-TMG-capped RNA-DNA chimeric oligonucleotide, which is expected to be conveyed to the nucleus by snurportin 1, a nuclear-transport protein. This 5'-TMG-capped RNA-DNA chimeric molecule was synthesized by the enzymatic condensation Of M(3)(2,2,7)G(5')pppAmpUmpA with A(5')ppd(CpTpTpApCpCpT) in the presence of RNA ligase. An inherent serious side reaction was disclosed in the 5'-adenylation of d(CpTpTpApCpCpT) on the solid support, but the use of an active ester intermediate as the pA donor gave an improved result. (C) 2003 Published by Elsevier Science Ltd.

引用

页码：1703 / 1707

页数：5

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