Circular oligonucleotides. Hybridization with 5S rRNA and digestion of RNA-DNA duplex with RNase H

被引：0
|
作者：
Fedorova, OA [1 ]
Gottikh, MB [1 ]
Romanova, EA [1 ]
Oretskaya, TS [1 ]
Dolinnaya, NG [1 ]
Shabarova, ZA [1 ]
机构：
[1] MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,RUSSIA
来源：
MOLECULAR BIOLOGY | 1995年 / 29卷 / 05期
关键词：
circular oligonucleotides; chemical ligation; RNase H;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chemical ligation was employed to synthesize circular oligonucleotides containing 1,2-dideoxy-D-ribofuranose inserts or nonmodified ones. Such oligonucleotides are able to form stable DNA duplexes with short oligonucleotides complementary to a part of the circular molecule. Circular oligonucleotides are able to interact with 120-unit long RNA; the hybrid duplexes formed may be attacked by RNase H.
引用
收藏
页码：689 / 693
页数：5
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