Circular oligonucleotides. Hybridization with 5S rRNA and digestion of RNA-DNA duplex with RNase H

被引：0

作者：

Fedorova, OA ^{[1
]}

Gottikh, MB ^{[1
]}

Romanova, EA ^{[1
]}

Oretskaya, TS ^{[1
]}

Dolinnaya, NG ^{[1
]}

Shabarova, ZA ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,RUSSIA

来源：

MOLECULAR BIOLOGY | 1995年 / 29卷 / 05期

关键词：

circular oligonucleotides; chemical ligation; RNase H;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemical ligation was employed to synthesize circular oligonucleotides containing 1,2-dideoxy-D-ribofuranose inserts or nonmodified ones. Such oligonucleotides are able to form stable DNA duplexes with short oligonucleotides complementary to a part of the circular molecule. Circular oligonucleotides are able to interact with 120-unit long RNA; the hybrid duplexes formed may be attacked by RNase H.

引用

页码：689 / 693

页数：5

共 23 条

[21] AUTORADIOGRAPHIC STUDY OF H-3 URIDINE INCORPORATION INTO NUCLEOLI AND DNA H-3 5S DNA INSITU HYBRIDIZATION IN ROOT-NODULES AND UNINFECTED LUPINUS-LUTEUS L TISSUES
OLSZEWSKA, MJ
KONONOWICZ, AK
MADRZAK, CJ
LEGOCKI, AB
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA, 1991, 29 (02): : 51 - &
[22] Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement
D C Cabral-de-Mello
R C Moura
C Martins
Heredity, 2010, 104 : 393 - 400
[23] Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement
Cabral-de-Mello, D. C.
Moura, R. C.
Martins, C.
HEREDITY, 2010, 104 (04) : 393 - 400

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