A TICT based two-photon fluorescent probe for cysteine and homocysteine in living cells

被引：42
|
作者：
Wei, Xinjie [1 ]
Yang, Xiuli [2 ]
Feng, Yan [1 ]
Ning, Peng [1 ]
Yu, Haizhu [1 ]
Zhu, Manzhou [1 ]
Xi, Xinguo [2 ]
Guo, Qingxiang [1 ,3 ]
Meng, Xiangming [1 ]
机构：
[1] Anhui Univ, Dept Chem, Hefei 230601, Peoples R China
[2] Yancheng Inst Technol, Jiangsu Collaborat Innovat Ctr Ecol Bldg Mat & En, Yancheng 224051, Peoples R China
[3] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 231卷
基金：
中国国家自然科学基金;
关键词：
TICT; Two-photon; Fluorescent probe; Cysteine; Homocysteine; INTRAMOLECULAR CHARGE-TRANSFER; HIGH SELECTIVITY; GLUTATHIONE; CHEMOSENSOR; HYDRAZINE; MECHANISM; NEURONS; DESIGN; THIOLS; IONS;
D O I：
10.1016/j.snb.2016.03.027
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A two-photon fluorescent probe (NQ) for cysteine (Cys) and homocysteine (Hcy) was developed based on twisted-intramolecular-charge-transfer (TICT) mechanism. NQshowed excellent "OFF-ON" fluorescence (at 540 nm) detection signal on addition of Cys/Hcy with high selectivity. The detection mechanism was successfully proved by theoretical calculations, viscosity dependent fluorescence and time resolved fluorescence spectra. NQ exhibits large two-photon absorption (TPA) cross-section with Cys/Hcy (580 and 710 GM) at nearly 800 nm, respectively. Cell-culture results indicated that NQ was cell-permeable and could be used to detect Cys/Hcy with low cytotoxicity under two-photon excitation in living cells. To our best knowledge, NQ is the first TICT based two-photon fluorescent probe for Cys/Hcy. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：285 / 292
页数：8
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