A TICT based two-photon fluorescent probe for cysteine and homocysteine in living cells

被引：44

作者：

Wei, Xinjie ^{[1
]}

Yang, Xiuli ^{[2
]}

Feng, Yan ^{[1
]}

Ning, Peng ^{[1
]}

Yu, Haizhu ^{[1
]}

Zhu, Manzhou ^{[1
]}

Xi, Xinguo ^{[2
]}

Guo, Qingxiang ^{[1
,3
]}

Meng, Xiangming ^{[1
]}

机构：

[1] Anhui Univ, Dept Chem, Hefei 230601, Peoples R China

[2] Yancheng Inst Technol, Jiangsu Collaborat Innovat Ctr Ecol Bldg Mat & En, Yancheng 224051, Peoples R China

[3] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 231卷

基金：

中国国家自然科学基金;

关键词：

TICT; Two-photon; Fluorescent probe; Cysteine; Homocysteine; INTRAMOLECULAR CHARGE-TRANSFER; HIGH SELECTIVITY; GLUTATHIONE; CHEMOSENSOR; HYDRAZINE; MECHANISM; NEURONS; DESIGN; THIOLS; IONS;

D O I：

10.1016/j.snb.2016.03.027

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A two-photon fluorescent probe (NQ) for cysteine (Cys) and homocysteine (Hcy) was developed based on twisted-intramolecular-charge-transfer (TICT) mechanism. NQshowed excellent "OFF-ON" fluorescence (at 540 nm) detection signal on addition of Cys/Hcy with high selectivity. The detection mechanism was successfully proved by theoretical calculations, viscosity dependent fluorescence and time resolved fluorescence spectra. NQ exhibits large two-photon absorption (TPA) cross-section with Cys/Hcy (580 and 710 GM) at nearly 800 nm, respectively. Cell-culture results indicated that NQ was cell-permeable and could be used to detect Cys/Hcy with low cytotoxicity under two-photon excitation in living cells. To our best knowledge, NQ is the first TICT based two-photon fluorescent probe for Cys/Hcy. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：285 / 292

页数：8

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