Automated analysis of polyethylene glycol-induced inhibition of P-glycoprotein activity in vitro

被引：21
|
作者：
Hugger, ED
Cole, CJ
Raub, TJ
Burton, PS
Borchardt, RT
机构：
[1] Univ Kansas, Dept Pharmaceut Chem, Lawrence, KS 66047 USA
[2] Pharmacia Corp, Drug Absorpt & Transport Grp, Kalamazoo, MI 49007 USA
来源：
JOURNAL OF PHARMACEUTICAL SCIENCES | 2003年 / 92卷 / 01期
关键词：
Caco-2; MDR1-MDCK; and wild-type MDCK cells; robotics; PEG-300; P-glycoprotein;
D O I：
10.1002/jps.10265
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Previous studies in our laboratories have shown that commonly used polyethoxylated pharmaceutical excipients inhibit P-glycoprotein activity in cell culture models of the intestinal mucosa. The results presented in this technical note show that the TECAN Genesis(TM) robotic workstation can be utilized to automate cellular transport studies for evaluating excipient effects on P-glycoprotein activity in vitro and for estimating the permeation of drug-like molecules across cell monolayers. (C) 2002 Wiley-Liss, Inc.
引用
收藏
页码：21 / 26
页数：6
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