Automated analysis of polyethylene glycol-induced inhibition of P-glycoprotein activity in vitro

被引：21
|
作者：
Hugger, ED
Cole, CJ
Raub, TJ
Burton, PS
Borchardt, RT
机构：
[1] Univ Kansas, Dept Pharmaceut Chem, Lawrence, KS 66047 USA
[2] Pharmacia Corp, Drug Absorpt & Transport Grp, Kalamazoo, MI 49007 USA
来源：
JOURNAL OF PHARMACEUTICAL SCIENCES | 2003年 / 92卷 / 01期
关键词：
Caco-2; MDR1-MDCK; and wild-type MDCK cells; robotics; PEG-300; P-glycoprotein;
D O I：
10.1002/jps.10265
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Previous studies in our laboratories have shown that commonly used polyethoxylated pharmaceutical excipients inhibit P-glycoprotein activity in cell culture models of the intestinal mucosa. The results presented in this technical note show that the TECAN Genesis(TM) robotic workstation can be utilized to automate cellular transport studies for evaluating excipient effects on P-glycoprotein activity in vitro and for estimating the permeation of drug-like molecules across cell monolayers. (C) 2002 Wiley-Liss, Inc.
引用
收藏
页码：21 / 26
页数：6
相关论文
共 50 条
  • [21] INHIBITION OF POLYETHYLENE GLYCOL-INDUCED CELL-FUSION OF HEP2 CELLS BY LECTINS
    FAN, VSC
    EALY, GT
    MCCAMMON, JR
    PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE, 1979, 161 (04): : 484 - 487
  • [22] INHIBITION OF P-GLYCOPROTEIN ACTIVITY INTERFERES WITH INTRACELLULAR STEROL TRANSPORT
    DEBRY, PW
    METHERALL, JE
    CLINICAL RESEARCH, 1994, 42 (01): : A85 - A85
  • [23] Inhibition of P-glycoprotein activity in human leukemic cells by mifepristone
    Fardel, O
    Courtois, A
    Drenou, B
    Lamy, T
    Lecureur, V
    lePrise, PY
    Fauchet, R
    ANTI-CANCER DRUGS, 1996, 7 (06) : 671 - 677
  • [24] Increase in doxorubicin cytotoxicity by carvedilol inhibition of P-glycoprotein activity
    Jonsson, O
    Behnam-Motlagh, P
    Persson, M
    Henriksson, R
    Grankvist, K
    BIOCHEMICAL PHARMACOLOGY, 1999, 58 (11) : 1801 - 1806
  • [25] Increase in doxorubicin cytotoxicity by inhibition of P-glycoprotein activity with lomerizine
    Shiraki, N
    Hamada, A
    Ohmura, T
    Tokunaga, J
    Oyama, N
    Nakano, M
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2001, 24 (05) : 555 - 557
  • [26] Polyethylene glycol-induced adverse reaction: an allergyc mechanism is suggested
    Carrillo Fernandez-Paredes, P.
    Boulaich, M.
    Lopez-Saez, M.
    de la Cruz Murie, P.
    Lopez Sanchez, J.
    Pagan Aleman, J.
    ALLERGY, 2012, 67 : 641 - 642
  • [27] GERMINATION OF SORGHUM BICOLOR UNDER POLYETHYLENE GLYCOL-INDUCED STRESS
    SAINTCLAIR, PM
    CANADIAN JOURNAL OF PLANT SCIENCE, 1976, 56 (01) : 21 - 24
  • [28] Influence of lipid lowering fibrates on P-glycoprotein activity in vitro
    Ehrhardt, M
    Lindenmaier, H
    Burhenne, J
    Haefeli, WE
    Weiss, J
    BIOCHEMICAL PHARMACOLOGY, 2004, 67 (02) : 285 - 292
  • [29] Influence of lipid lowering fibrates on P-glycoprotein activity in vitro
    Ehrhardt, M
    Weiss, J
    Burhenne, J
    Haefeli, WE
    EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, 2002, 58 (07) : S80 - S80
  • [30] POLYETHYLENE GLYCOL-INDUCED TRANSPLANTATION OF CHLOROPLASTS INTO PROTOPLASTS - ULTRASTRUCTURAL ASSESSMENT
    DAVEY, MR
    FREARSON, EM
    POWER, JB
    PLANT SCIENCE LETTERS, 1976, 7 (01): : 7 - 16
12345