PI/DAPI Fluorochrome Staining Technique for Identifying Citrus Chromosomes

被引：0
|
作者：
Yu Yang [1 ]
Wang Wei [1 ]
Yang Xiao-ling [1 ]
机构：
[1] Tongji Univ, Coll Life Sci & Technol, Shanghai 200092, Peoples R China
来源：
2009 ACADEMIC CONFERENCE ON HORTICULTURE SCIENCE AND TECHNOLOGY, PROCEEDINGS | 2009年
关键词：
PI/DAPI staining; chromosome; citrus; karyotype; CMA BANDING-PATTERNS; IDENTIFICATION; VARIABILITY; PONCIRUS;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
The mitotic metaphase chromosomes of several citrus were stained with propidium iodide (PI) and (DAPI). The stable chromosome patterns were observed with the suitable proportion of PI/DAPI volume. Based on the relative-sized PI signals and the morphology of no-signal chromosomes, 18 chromosomes of citrus were classified into 7 groups. In addition, the relative-sized PI signals on the chromosomes were observed maintain stable in the cell cycle. It is considered that the chromosome combined with PI fluorochrome were highly stable construction.
引用
收藏
页码：53 / 55
页数：3
相关论文
共 50 条
  • [31] A new chromosome fluorescence banding technique combining DAPI staining with image analysis in plants
    Liu, JY
    She, CW
    Hu, ZL
    Xiong, ZY
    Liu, LH
    Song, YC
    CHROMOSOMA, 2004, 113 (01) : 16 - 21
  • [32] Phenomenon of selective staining of pericentromeric heterochromatin regions in chromosomes from spontaneously dividing cells by the acridine orange fluorochrome
    Kuznetzova, T. V.
    Trofimova, I. L.
    Lyapunova, M. S.
    Evdokimenko, E. V.
    Baranov, V. S.
    RUSSIAN JOURNAL OF GENETICS, 2012, 48 (04) : 369 - 375
  • [33] EXPRESSION OF HETEROCHROMATIN BY RESTRICTION ENDONUCLEASE TREATMENT AND DISTAMYCIN A/DAPI STAINING OF INDIAN MUNTJAC (MUNTIACUS-MUNTJAK) CHROMOSOMES
    BABU, A
    VERMA, RS
    CYTOGENETICS AND CELL GENETICS, 1986, 41 (02): : 96 - 100
  • [34] Phenomenon of selective staining of pericentromeric heterochromatin regions in chromosomes from spontaneously dividing cells by the acridine orange fluorochrome
    T. V. Kuznetzova
    I. L. Trofimova
    M. S. Lyapunova
    E. V. Evdokimenko
    V. S. Baranov
    Russian Journal of Genetics, 2012, 48 : 369 - 375
  • [35] Silver staining as a new banding technique to identify aphid chromosomes
    Gian Carlo Manicardi
    Davide Bizzaro
    Mauro Mandrioli
    Umberto Bianchi
    Chromosome Research, 1998, 6 : 55 - 57
  • [36] NEW SELECTIVE GIEMSA TECHNIQUE FOR HUMAN CHROMOSOMES, CD STAINING
    EIBERG, H
    NATURE, 1974, 248 (5443) : 55 - 55
  • [37] STAINING OF ULTRAVIOLET IRRADIATED CHROMOSOMES BY GOMORI ALKALINE PHOSPHATASE TECHNIQUE
    AMENTA, PS
    STAIN TECHNOLOGY, 1961, 36 (01): : 15 - &
  • [38] Silver staining as a new banding technique to identify aphid chromosomes
    Manicardi, GC
    Bizzaro, D
    Mandrioli, M
    Bianchi, U
    CHROMOSOME RESEARCH, 1998, 6 (01) : 55 - 57
  • [39] rDNA sites and heterochromatin in Meiwa kumquat (Fortunella crassifolia Swing.) chromosomes revealed by FISH and CMA/DAPI staining
    Miranda, M
    Ikeda, F
    Endo, T
    Moriguchi, T
    Omura, M
    CARYOLOGIA, 1997, 50 (3-4) : 333 - 340
  • [40] MODIFIED C-BAND TECHNIQUE FOR STAINING OF DIFFUSE DIPLOTENE CHROMOSOMES
    KLASTERSKA, I
    NATARAJAN, AT
    STAIN TECHNOLOGY, 1976, 51 (03): : 209 - 211
12345