PI/DAPI Fluorochrome Staining Technique for Identifying Citrus Chromosomes

被引：0
|
作者：
Yu Yang [1 ]
Wang Wei [1 ]
Yang Xiao-ling [1 ]
机构：
[1] Tongji Univ, Coll Life Sci & Technol, Shanghai 200092, Peoples R China
来源：
2009 ACADEMIC CONFERENCE ON HORTICULTURE SCIENCE AND TECHNOLOGY, PROCEEDINGS | 2009年
关键词：
PI/DAPI staining; chromosome; citrus; karyotype; CMA BANDING-PATTERNS; IDENTIFICATION; VARIABILITY; PONCIRUS;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
The mitotic metaphase chromosomes of several citrus were stained with propidium iodide (PI) and (DAPI). The stable chromosome patterns were observed with the suitable proportion of PI/DAPI volume. Based on the relative-sized PI signals and the morphology of no-signal chromosomes, 18 chromosomes of citrus were classified into 7 groups. In addition, the relative-sized PI signals on the chromosomes were observed maintain stable in the cell cycle. It is considered that the chromosome combined with PI fluorochrome were highly stable construction.
引用
收藏
页码：53 / 55
页数：3
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