Endometrial cancer is the most abundant female gynecologic malignancy, ranking fourth in incidence among invasive tumors in women. Females of the BDII inbred rat strain are extremely prone to endometrial adenocarcinoma (EAC), and approximately 90% of virgin females spontaneously develop EAC during their lifetime. Thus, these rats serve as a useful model for the genetic analysis of this malignancy. In the present work, gene expression profiling, by means of cDNA microarrays, was performed on cDNA from endometrial tumor cell lines and from cell lines derived from nonmalignant lesions/normal tissues of the endometrium. We identified several genes associated with the transforming growth factor-beta (TGF-beta) pathway to be differentially expressed between endometrial tumor cell lines and nonmalignant lesions by using clustering and statistical inference analyses. The expression levels of the genes involved in the TGF-beta pathway were independently verified using semi quantitative reverse-transcription polymerase chain reaction. Repressed TGF-beta signaling has been reported previously in EAC carcinogenesis, but this is the first report demonstrating aberrations in the expression of TGF-beta downstream target genes. We propose that the irregularities present in TGF-beta pathway among the majority of the EAC tumor cell lines may affect EAC carcinogenesis. (c) 2007 Elsevier Inc. All rights reserved.
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Mashhad Univ Med Sci, Dept Clin Biochem, Fac Med, Mashhad 1394491388, Razavi Khorasan, Iran
Mashhad Univ Med Sci, Student Res Comm, Fac Med, Mashhad, Razavi Khorasan, IranMashhad Univ Med Sci, Dept Clin Biochem, Fac Med, Mashhad 1394491388, Razavi Khorasan, Iran
Soleimani, Atena
Pashirzad, Mehran
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Mashhad Univ Med Sci, Dept Clin Biochem, Fac Med, Mashhad 1394491388, Razavi Khorasan, IranMashhad Univ Med Sci, Dept Clin Biochem, Fac Med, Mashhad 1394491388, Razavi Khorasan, Iran
Pashirzad, Mehran
Avan, Amir
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Mashhad Univ Med Sci, Metab Syndrome Res Ctr, Mashhad, Razavi Khorasan, Iran
Mashhad Univ Med Sci, Dept Modern Sci & Technol, Fac Med, Mashhad, Razavi Khorasan, IranMashhad Univ Med Sci, Dept Clin Biochem, Fac Med, Mashhad 1394491388, Razavi Khorasan, Iran
Avan, Amir
Ferns, Gordon A.
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Brighton & Sussex Med Sch, Div Med Educ, Brighton, E Sussex, EnglandMashhad Univ Med Sci, Dept Clin Biochem, Fac Med, Mashhad 1394491388, Razavi Khorasan, Iran
Ferns, Gordon A.
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Khazaei, Majid
Hassanian, Seyed Mahdi
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Mashhad Univ Med Sci, Dept Clin Biochem, Fac Med, Mashhad 1394491388, Razavi Khorasan, Iran
Mashhad Univ Med Sci, Metab Syndrome Res Ctr, Mashhad, Razavi Khorasan, IranMashhad Univ Med Sci, Dept Clin Biochem, Fac Med, Mashhad 1394491388, Razavi Khorasan, Iran