Cloning, sequencing and polymorphism analyzing of the exon 4 of the kappa-casein gene in yak

被引：0

作者：

Fan, BL

Li, N ^{[1
]}

Hu, XX

Wu, CX

机构：

[1] China Agr Univ, State Key Labs Agrobiotechnol, Beijing 100094, Peoples R China

[2] China Agr Univ, Coll Anim Sci & Technol, Beijing 100094, Peoples R China

[3] Hebei Handan Agr Coll, Handan 057150, Peoples R China

来源：

PROGRESS IN NATURAL SCIENCE | 2000年 / 10卷 / 10期

关键词：

yak; kappa-casein gene; exon; 4; polymorphism;

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

According to the nucleotide sequence of cattle kappa-casein gene, a pair of primers was synthesized to amplify the sequences of the exon 4 and partial intron 4 of tills gene in yak. The restriction fragment length polymorphisms were identified by the digestions of the PCR product with Hind In or Pst I in both populations of cattle and yak, but the frequency of the allele and the genotype were significantly different between the two animal populations.

引用

页码：769 / 773

页数：5

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