Kappa-casein gene polymorphism in Indian goats

The present work was carried out to analyse the kappa-casein variants in five Indian goat breeds by SDS-PAGE, PCR-RFLP and SSCP method. A total of 152 unrelated blood samples and 102 milk samples belonging to Barbari, Beetal, Marwari, Surd and Local MP (non-descript) goats were used for analysis. SDS-PAGE exhibited kappa-casein allele A in all the breeds. Moreover PCR+RFLP analysis also confirmed the presence of AA genotype in all the breeds. PCR-SSCP analysis of kappa-casein genetic variant showed the kappa-casein A and B allele in the analysed samples. The kappa-casein A allele was the dominant variant found in all analyzed breeds with frequencies ranging from 0.70 (Barbari) to 0.8 (Local MP). The variant B was most frequent in Barbari goats as compared to other breeds.