Cloning, sequencing and polymorphism analyzing of the exon 4 of the kappa-casein gene in yak

被引:0
|
作者
Fan, BL
Li, N [1 ]
Hu, XX
Wu, CX
机构
[1] China Agr Univ, State Key Labs Agrobiotechnol, Beijing 100094, Peoples R China
[2] China Agr Univ, Coll Anim Sci & Technol, Beijing 100094, Peoples R China
[3] Hebei Handan Agr Coll, Handan 057150, Peoples R China
关键词
yak; kappa-casein gene; exon; 4; polymorphism;
D O I
暂无
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
According to the nucleotide sequence of cattle kappa-casein gene, a pair of primers was synthesized to amplify the sequences of the exon 4 and partial intron 4 of tills gene in yak. The restriction fragment length polymorphisms were identified by the digestions of the PCR product with Hind In or Pst I in both populations of cattle and yak, but the frequency of the allele and the genotype were significantly different between the two animal populations.
引用
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页码:769 / 773
页数:5
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