A pH sensitive fluorescent cyanine dye for biological applications

被引：101
|
作者：
Briggs, MS [1 ]
Burns, DD [1 ]
Cooper, ME [1 ]
Gregory, SJ [1 ]
机构：
[1] Amersham Pharmacia Biotech, Drug Discovery, Forest Farm, Cardiff CF14 7YT, S Glam, Wales
来源：
CHEMICAL COMMUNICATIONS | 2000年 / 23期
关键词：
D O I：
10.1039/b007108k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A pentamethine cyanine dye has been synthesised that is fluorescent when protonated, becoming non-fluorescent upon proton abstraction. The probe has a pK(a) of 7.5, with observable changes in the fluorescent emission properties across a pH range of 6.0-8.0, therefore providing a useful probe for studying pH in biological media.
引用
收藏
页码：2323 / 2324
页数：2
相关论文
共 50 条
  • [41] pH-Sensitive Fluorescent Dyes: Are They Really pH-Sensitive in Cells?
    Zhang, Xiao-Xiang
    Wang, Zhe
    Yue, Xuyi
    Ma, Ying
    Kiesewetter, Dale O.
    Chen, Xiaoyuan
    MOLECULAR PHARMACEUTICS, 2013, 10 (05) : 1910 - 1917
  • [42] Polylysine Crosslinked AIE Dye Based Fluorescent Organic Nanoparticles for Biological Imaging Applications
    Liu, Meiying
    Zhang, Xiqi
    Yang, Bin
    Liu, Liangji
    Deng, Fengjie
    Zhang, Xiaoyong
    Wei, Yen
    MACROMOLECULAR BIOSCIENCE, 2014, 14 (09) : 1260 - 1267
  • [43] pH-sensitive Itaconic acid based polymeric hydrogels for dye removal applications
    Sakthivel, M.
    Franklin, D. S.
    Guhanathan, S.
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2016, 134 : 427 - 432
  • [44] Novel fluorescent trimethine cyanine dye 7519 for amyloid fibril inhibition assay
    Volkova, K. D.
    Kovalska, V. B.
    Inshin, D.
    Slominskii, Y. L.
    Tolmachev, O. I.
    Yarmoluk, S. M.
    BIOTECHNIC & HISTOCHEMISTRY, 2011, 86 (03) : 188 - 191
  • [45] A novel pH sensitive water soluble fluorescent nanomicellar sensor for potential biomedical applications
    Georgiev, Nikolai I.
    Bryaskova, Rayna
    Tzoneva, Rumiana
    Ugrinova, Iva
    Detrembleur, Christophe
    Miloshev, Stoyan
    Asiri, Abdullah M.
    Qusti, Abdullah H.
    Bojinov, Vladimir B.
    BIOORGANIC & MEDICINAL CHEMISTRY, 2013, 21 (21) : 6292 - 6302
  • [46] Cyanine dye-protein interactions: Looking for fluorescent probes for amyloid structures
    Volkova, K. D.
    Kovalska, V. B.
    Balanda, A. O.
    Vermeij, R. J.
    Subramaniam, V.
    Slominskii, Yu. L.
    Yarmoluk, S. M.
    JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2007, 70 (05): : 727 - 733
  • [47] USE OF A FLUORESCENT CYANINE DYE FOR ELECTRO-PHYSIOLOGICAL STUDIES ON THE FROG CORNEA
    GRAVES, CN
    SACHS, G
    REHM, WS
    AMERICAN JOURNAL OF PHYSIOLOGY, 1980, 238 (01): : C21 - C26
  • [48] Spectroscopic Studies of α,γ-Disubstituted Trimethine Cyanine: New Fluorescent Dye for Nucleic Acids
    I. V. Valyukh
    V. B. Kovalska
    Y. L. Slominskii
    S. M. Yarmoluk
    Journal of Fluorescence, 2002, 12 : 105 - 107
  • [49] Spectroscopic studies of α,γ-disubstituted trimethine cyanine:: New fluorescent dye for nucleic acids
    Valyukh, IV
    Kovalska, VB
    Slominskii, YL
    Yarmoluk, SM
    JOURNAL OF FLUORESCENCE, 2002, 12 (01) : 105 - 107
  • [50] Detection of acid moisture in photovoltaic modules using a dual wavelength pH-sensitive fluorescent dye
    Asaka, Takashi
    Iwami, Kentaro
    Taguchi, Atsushi
    Umeda, Norihiro
    Masuda, Atsushi
    JAPANESE JOURNAL OF APPLIED PHYSICS, 2014, 53 (04)
12345