Evaluation of an automated extraction method for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Cobas Amplicor PCR from different sample materials

被引：4
|
作者：
Geertsen, Ralf
Friderich, Paul
Dobec, Marinko
Emler, Stefan
机构：
[1] Inst Vir Ltd, Dept Mol Biol, CH-8803 Ruschlikon, Switzerland
[2] Med Lab Dr F Kaeppeli AG, Zurich, Switzerland
[3] Smartgene GmbH, Zug, Switzerland
来源：
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES | 2007年 / 39卷 / 05期
关键词：
D O I：
10.1080/00365540601087582
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A commercially available automated device (MagNA Pure LCTM) was adapted for nucleic acid extraction of urogenital specimen for subsequent PCR detection of Chlamydia trachomatis and Neisseria gonorrhoeae in a clinical laboratory. Results were compared to the standard manual extraction procedure and showed excellent correlation, with even slightly increased sensitivity.
引用
收藏
页码：405 / 408
页数：4
相关论文
共 50 条
  • [1] Detection of male genital infection with Chlamydia trachomatis and Neisseria gonorrhoeae using an automated multiplex PCR system (Cobas® Amplicor™)
    Higgins, SP
    Klapper, PE
    Struthers, JK
    Bailey, AS
    Gough, AP
    Moore, R
    Corbitt, G
    Bhattacharyya, MN
    [J]. INTERNATIONAL JOURNAL OF STD & AIDS, 1998, 9 (01) : 21 - 24
  • [2] Evaluation of COBAS AMPLICOR (Roche):: Accuracy in detection of Chlamydia trachomatis and Neisseria gonorrhoeae by coamplification of endocervical specimens
    Livengood, CH
    Wrenn, JW
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (08) : 2928 - 2932
  • [3] Evaluation of magnapure extractions for screening of Chlamydia trachomatis and Neisseria gonorrhoeae in the Roche COBAS amplicor assay
    Montalvan, I.
    Shah, M.
    Lam, X.
    Millard, C.
    Hutchinson, A.
    Fernandes, H.
    [J]. JOURNAL OF MOLECULAR DIAGNOSTICS, 2006, 8 (05): : 668 - 668
  • [4] High frequency of competitive inhibition in the Roche Cobas AMPLICOR multiplex PCR for Chlamydia trachomatis and Neisseria gonorrhoeae
    Hamilton, MS
    Otto, M
    Nickell, A
    Abel, D
    Ballam, Y
    Schremmer, R
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) : 4393 - 4393
  • [5] Cobas® 4800: a fully automated system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae
    Van der Pol, Barbara
    [J]. EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2013, 13 (02) : 131 - 140
  • [6] Multicenter evaluation of the AMPLICOR and automated COBAS AMPLICOR CT/NG tests for detection of Chlamydia trachomatis
    Van der Pol, B
    Quinn, TC
    Gaydos, CA
    Crotchfelt, K
    Schachter, J
    Moncada, J
    Jungkind, D
    Martin, DH
    Turner, B
    Peyton, C
    Jones, RB
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) : 1105 - 1112
  • [7] Multicenter evaluation of the fully automated COBAS AMPLICOR PCR test for detection of Chlamydia trachomatis in urogenital specimens
    Vincelette, J
    Schirm, J
    Bogard, M
    Bourgault, AM
    Luijt, DS
    Bianchi, A
    Vader, PCV
    Butcher, A
    Rosenstraus, M
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (01) : 74 - 80
  • [8] Evaluation of conventional and real-time PCR assays using two targets for confirmation of results of the COBAS AMPLICOR Chlamydia trachomatis Neisseria gonorrhoeae test for detection of Neisseria gonorrhoeae in clinical samples
    Boel, CHE
    van Herk, CMC
    Berretty, PJM
    Onland, GHW
    van den Brule, AJC
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (05) : 2231 - 2235
  • [9] Multicenter evaluation of AMPLICOR and automated COBAS AMPLICOR CT/NG tests for Neisseria gonorrhoeae
    Martin, DH
    Cammarata, C
    van der Pol, B
    Jones, RB
    Quinn, TC
    Gaydos, CA
    Crotchfelt, K
    Schachter, J
    Moncada, J
    Jungkind, D
    Turner, B
    Peyton, C
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (10) : 3544 - 3549
  • [10] EVALUATION OF THE ROCHE COBAS 4800 FOR THE DETECTION OF CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOEAE USING ENDOCERVICAL SPECIMENS
    Pantone, A.
    Van der Pol, B.
    Lillis, R.
    Loyd, B.
    Nye, M. B.
    Taylor, S.
    [J]. SEXUALLY TRANSMITTED INFECTIONS, 2011, 87 : A278 - A279
12345