Expression of biologically active human calpastatin in baculovirus-infected insect cells and in Escherichia coli

被引：22
|
作者：
Hitomi, K [1 ]
Yokoyama, A [1 ]
Maki, M [1 ]
机构：
[1] Nagoya Univ, Sch Agr Sci, Nagoya, Aichi 46401, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 01期
关键词：
calpain; calpastatin; protease; baculovirus;
D O I：
10.1271/bbb.62.136
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Calpastatin, an endogeneous inhibitor protein acting on calpain (Ca2+-dependent cysteine proteinase), is widely distributed in animal tissues and cells. Two different expression systems, baculovirus-infected Spodoptera frugiperda (Sf9) insect cells and Escherichia coli, were used for overexpression of the human calpastatin tagged with N-terminal hexahistidine peptide. Recombinant calpastatin was purified to homogeneity by nickel ion affinity chromatography and gel filtration separation. Purified recombinant proteins from both systems have similar inhibitory activity for calpain.
引用
收藏
页码：136 / 141
页数：6
相关论文
共 50 条
  • [31] Overexpression of PNGase At from baculovirus-infected insect cells
    Paquin, NF
    Tarentino, AL
    Plummer, TH
    PROTEIN EXPRESSION AND PURIFICATION, 1998, 14 (02) : 302 - 308
  • [32] Expression and characterization of recombinant alpha-galactosidase in baculovirus-infected insect cells
    Zhu, A
    Wang, ZK
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 235 (1-2): : 332 - 337
  • [33] Mammalian glycosyltransferase expression allows sialoglycoprotein production by baculovirus-infected insect cells
    Seo, NS
    Hollister, JR
    Jarvis, DL
    PROTEIN EXPRESSION AND PURIFICATION, 2001, 22 (02) : 234 - 241
  • [34] EXPRESSION OF FUNCTIONAL METABOTROPIC AND IONOTROPIC GLUTAMATE RECEPTORS IN BACULOVIRUS-INFECTED INSECT CELLS
    ROSS, SM
    TAVERNA, FA
    PICKERING, DS
    WANG, LY
    MACDONALD, JF
    PENNEFATHER, PS
    HAMPSON, DR
    NEUROSCIENCE LETTERS, 1994, 173 (1-2) : 139 - 142
  • [35] Screening family C GPCRs for heterologous expression in baculovirus-infected insect cells
    Tyssowski, Kelsey
    Olson, Rich
    FASEB JOURNAL, 2011, 25
  • [36] HUMAN PREPROENDOTHELIN-1 IS CONVERTED INTO ACTIVE ENDOTHELIN-1 BY BACULOVIRUS-INFECTED INSECT CELLS
    BENATTI, L
    COZZI, L
    ZAMAI, M
    TAMBURIN, M
    VAGHI, F
    CAIOLFA, VR
    FABBRINI, MS
    SARMIENTOS, P
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 186 (02) : 753 - 759
  • [37] ANTIBODY-PRODUCTION IN BACULOVIRUS-INFECTED INSECT CELLS
    PUTLITZ, JZ
    KUBASEK, WL
    DUCHENE, M
    MARGET, M
    VONSPECHT, BU
    DOMDEY, H
    BIO-TECHNOLOGY, 1990, 8 (07): : 651 - 654
  • [38] Understanding oxidative stress in baculovirus-infected insect cells
    Bond, E
    Chauhan, G
    Turner, R
    Doherty, K
    Murhammer, D
    FREE RADICAL BIOLOGY AND MEDICINE, 2005, 39 : S23 - S24
  • [39] HUMAN MACROPHAGE-COLONY-STIMULATING FACTOR (HMCSF) EXPRESSED IN BACULOVIRUS-INFECTED INSECT CELLS IS BIOLOGICALLY-ACTIVE IN ITS MONOMERIC FORM
    QIU, P
    LI, J
    QIN, JC
    XI, T
    ZHU, D
    BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, 1995, 35 (04): : 773 - 779
  • [40] Purification and characterization of secreted human leptin produced in baculovirus-infected insect cells
    Churgay, LM
    Kovacevic, S
    Tinsley, FC
    Kussow, CM
    Millican, RL
    Miller, JR
    Hale, JE
    GENE, 1997, 190 (01) : 131 - 137
12345