Expression of biologically active human calpastatin in baculovirus-infected insect cells and in Escherichia coli

被引：22
|
作者：
Hitomi, K [1 ]
Yokoyama, A [1 ]
Maki, M [1 ]
机构：
[1] Nagoya Univ, Sch Agr Sci, Nagoya, Aichi 46401, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 01期
关键词：
calpain; calpastatin; protease; baculovirus;
D O I：
10.1271/bbb.62.136
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Calpastatin, an endogeneous inhibitor protein acting on calpain (Ca2+-dependent cysteine proteinase), is widely distributed in animal tissues and cells. Two different expression systems, baculovirus-infected Spodoptera frugiperda (Sf9) insect cells and Escherichia coli, were used for overexpression of the human calpastatin tagged with N-terminal hexahistidine peptide. Recombinant calpastatin was purified to homogeneity by nickel ion affinity chromatography and gel filtration separation. Purified recombinant proteins from both systems have similar inhibitory activity for calpain.
引用
收藏
页码：136 / 141
页数：6
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