Rif1 originally recognized for its role at telomeres in budding yeast, has been implicated in a wide variety of cellular processes in mammals, including pluripotency of stem cells, response to double-strand breaks, and breast cancer development. As the molecular function of Rif1 is not known, we examined the consequences of Rif1 deficiency in mouse cells. Rif1 deficiency leads to failure in embryonic development, and conditional deletion of Rif1 from mouse embryo fibroblasts affects S-phase progression, rendering cells hypersensitive to replication poisons. Rif1 deficiency does not alter the activation of the DNA replication checkpoint but rather affects the execution of repair. RNA interference to human Rif1 decreases the efficiency of homology-directed repair (HDR), and Rif1 deficiency results in aberrant aggregates of the HDR factor Rad51. Consistent with a role in S-phase progression, Rif1 accumulates at stalled replication forks, preferentially around pericentromeric heterochromatin. Collectively, these findings reveal a function for Rif1 in the repair of stalled forks by facilitating HDR.
机构:
Mem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USAMem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
Nakanishi, Koji
Cavallo, Francesca
论文数: 0引用数: 0
h-index: 0
机构:
Univ Roma Tor Vergata, Dept Publ Hlth & Cell Biol, Sect Anat, Rome, ItalyMem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
Cavallo, Francesca
Perrouault, Loic
论文数: 0引用数: 0
h-index: 0
机构:
Museum Natl Hist Nat, F-75231 Paris, France
CNRS, UMR7196, Paris, France
INSERM, U565, Paris, FranceMem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
Perrouault, Loic
Giovannangeli, Carine
论文数: 0引用数: 0
h-index: 0
机构:
Museum Natl Hist Nat, F-75231 Paris, France
CNRS, UMR7196, Paris, France
INSERM, U565, Paris, FranceMem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
Giovannangeli, Carine
Moynahan, Mary Ellen
论文数: 0引用数: 0
h-index: 0
机构:
Mem Sloan Kettering Canc Ctr, Dept Med, New York, NY 10021 USAMem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
Moynahan, Mary Ellen
Barchi, Marco
论文数: 0引用数: 0
h-index: 0
机构:
Univ Roma Tor Vergata, Dept Publ Hlth & Cell Biol, Sect Anat, Rome, ItalyMem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
Barchi, Marco
Brunet, Erika
论文数: 0引用数: 0
h-index: 0
机构:
Mem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
Museum Natl Hist Nat, F-75231 Paris, France
CNRS, UMR7196, Paris, France
INSERM, U565, Paris, FranceMem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
Brunet, Erika
Jasin, Maria
论文数: 0引用数: 0
h-index: 0
机构:
Mem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USAMem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
机构:
Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Biochem, Chengdu, Peoples R ChinaSichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Biochem, Chengdu, Peoples R China
Zheng, Yanjiang
VanDusen, Nathan J.
论文数: 0引用数: 0
h-index: 0
机构:
Boston Childrens Hosp, Dept Cardiol, Boston, MA USASichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Biochem, Chengdu, Peoples R China
VanDusen, Nathan J.
Butler, Catalina E.
论文数: 0引用数: 0
h-index: 0
机构:
Boston Childrens Hosp, Dept Cardiol, Boston, MA USASichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Biochem, Chengdu, Peoples R China
Butler, Catalina E.
Ma, Qing
论文数: 0引用数: 0
h-index: 0
机构:
Boston Childrens Hosp, Dept Cardiol, Boston, MA USASichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Biochem, Chengdu, Peoples R China
Ma, Qing
King, Justin S.
论文数: 0引用数: 0
h-index: 0
机构:
Boston Childrens Hosp, Dept Cardiol, Boston, MA USASichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Biochem, Chengdu, Peoples R China
King, Justin S.
Pu, William T.
论文数: 0引用数: 0
h-index: 0
机构:
Boston Childrens Hosp, Dept Cardiol, Boston, MA USA
Harvard Stem Cell Inst, Cambridge, MA USASichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Biochem, Chengdu, Peoples R China
机构:
Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, Copenhagen, DenmarkUniv Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, Copenhagen, Denmark
Ochs, Fena
Somyajit, Kumar
论文数: 0引用数: 0
h-index: 0
机构:
Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, Copenhagen, DenmarkUniv Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, Copenhagen, Denmark
Somyajit, Kumar
论文数: 引用数:
h-index:
机构:
Altmeyer, Matthias
Rask, Maj-Britt
论文数: 0引用数: 0
h-index: 0
机构:
Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, Copenhagen, DenmarkUniv Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, Copenhagen, Denmark
Rask, Maj-Britt
Lukas, Jiri
论文数: 0引用数: 0
h-index: 0
机构:
Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, Copenhagen, DenmarkUniv Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, Copenhagen, Denmark