Crystallization and preliminary X-ray crystallographic analysis of human geranylgeranyl pyrophosphate synthase

被引：0
|
作者：
Chen Yushu [1 ]
Li Xiaofeng [1 ]
Lou Zhiyong [1 ]
Wang Weina [1 ]
Pang Hai [1 ]
Rao Zihe [1 ]
机构：
[1] Tsing Hua Univ, Struct Biol Lab, Beijing 100084, Peoples R China
来源：
PROGRESS IN NATURAL SCIENCE | 2006年 / 16卷 / 12期
关键词：
human geranylgeranyl pyrophosphate synthase; crystallization; spacegroup; unit-cell parameter;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Human geranylgeranyl pyrophosphate synthase (GGPS) is an enzyme that catalyzes the synthesis of geranylgeranyl pyrophosphate (GGPP) from farnesyldiphosphate and isopentenyldiphosphate. Recombinant human GGPS was crystallized by the hangingdrop vapor diffusion method. Crystals were grown at 18 degrees C using PEG 4000 as precipitant. Diffraction data were obtained to a resolution of 2.8 angstrom from a single frozen crystal belonging to space group P1, with unit-cell parameters: a = 68. 9 angstrom, b 107.7 angstrom, c - 137.4 angstrom, alpha=99.6 degrees beta= 97.6 degrees, gamma=97.8 degrees.
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收藏
页码：1353 / 1356
页数：4
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