Crystallization and preliminary X-ray crystallographic studies on recombinant human carnitine acetyltransferase

被引：4
|
作者：
Lian, W
Govindasamy, L
Gu, YR
Kukar, T
Agbandje-McKenna, M
McKenna, R
Wu, DH [1 ]
机构：
[1] McKnight Brain Inst, Dept Med Chem, Gainesville, FL 32610 USA
[2] Univ Florida, Gainesville, FL 32610 USA
[3] McKnight Brain Inst, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷
关键词：
D O I：
10.1107/S0907444902005498
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this paper, the purification, crystallization and preliminary X-ray crystallographic studies of human carnitine acetyltransferase are reported. Recombinant human carnitine acetyltransferase crystals were grown by the hanging-drop vapor-diffusion method and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 137.65, b = 84.76, c = 57.65 Angstrom and one molecule per asymmetric unit. The intensity data were collected from a cryocooled crystal to 1.6 Angstrom resolution using a conventional X-ray source.
引用
收藏
页码：1193 / 1194
页数：2
相关论文
共 50 条
  • [1] Crystallization and preliminary X-ray crystallographic studies of human cyclophilin
    Hu, H
    Huang, CQ
    Liu, HL
    Han, Y
    Yu, L
    Bi, RC
    [J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2005, 61 : 216 - 218
  • [2] Crystallization and preliminary X-ray crystallograph is studies on recombinant rat choline acetyltransferase
    Lian, W
    Gu, YR
    Pedersen, B
    Kukar, T
    Govindasamy, L
    Agbandje-McKenna, M
    Jin, SG
    McKenna, R
    Wu, DH
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 374 - 375
  • [3] Crystallization and preliminary X-ray crystallographic studies of recombinant bovine neurocalcin delta
    Kumar, VD
    Hidaka, H
    Okazaki, K
    VijayKumar, S
    [J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1996, 25 (02): : 261 - 264
  • [4] Crystallization and preliminary X-ray crystallographic studies of human FAIM protein
    Gu, Jiang
    Ji, Xiaowei
    Qi, Jianxun
    Ma, Ying
    Mao, Xuhu
    Zou, Quanming
    [J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 929 - 931
  • [5] Crystallization and preliminary X-ray crystallographic studies of human autocrine motility factor
    Uemura, H
    Tanaka, N
    Funasaka, T
    Haga, A
    Nagase, H
    Raz, A
    Nakamura, KT
    [J]. PROTEIN AND PEPTIDE LETTERS, 2001, 8 (04): : 317 - 322
  • [6] Crystallization and preliminary X-ray crystallographic studies of recombinant human betaine-homocysteine S-methyltransferase
    Bose, N
    Momany, C
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 : 431 - 433
  • [7] Crystallization and preliminary X-ray crystallographic studies of rice α-galactosidase
    Fujimoto, Z
    Kobayashi, O
    Kaneko, S
    Momma, M
    Kobayashi, H
    Mizuno, H
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 : 1374 - 1375
  • [8] Crystallization and preliminary X-ray crystallographic analysis of human autotaxin
    Inoue, Keigo
    Tanaka, Nobutada
    Haga, Arayo
    Yamasaki, Kyohei
    Umeda, Tomonobu
    Kusakabe, Yoshio
    Sakamoto, Yasumitsu
    Nonaka, Takamasa
    Deyashiki, Yoshihiro
    Nakamura, Kazuo T.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 450 - 453
  • [9] Crystallization and preliminary X-ray diffraction studies of recombinant staphylokinase
    Zhan, CH
    Wan, ZL
    Chang, WR
    Yue, JJ
    Liang, DC
    Tang, QQ
    Gu, YL
    Zhang, XX
    Xu, G
    Zhu, YS
    Song, HY
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1996, 52 : 564 - 565
  • [10] Purification, crystallization and preliminary X-ray crystallographic studies on acetolactate decarboxylase
    Najmudin, S
    Andersen, JT
    Patkar, SA
    Borchert, TV
    Crout, DHG
    Fülöp, V
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2003, 59 : 1073 - 1075
12345