Crystallization and preliminary X-ray crystallographic studies on recombinant human carnitine acetyltransferase

被引:4
|
作者
Lian, W
Govindasamy, L
Gu, YR
Kukar, T
Agbandje-McKenna, M
McKenna, R
Wu, DH [1 ]
机构
[1] McKnight Brain Inst, Dept Med Chem, Gainesville, FL 32610 USA
[2] Univ Florida, Gainesville, FL 32610 USA
[3] McKnight Brain Inst, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA
关键词
D O I
10.1107/S0907444902005498
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this paper, the purification, crystallization and preliminary X-ray crystallographic studies of human carnitine acetyltransferase are reported. Recombinant human carnitine acetyltransferase crystals were grown by the hanging-drop vapor-diffusion method and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 137.65, b = 84.76, c = 57.65 Angstrom and one molecule per asymmetric unit. The intensity data were collected from a cryocooled crystal to 1.6 Angstrom resolution using a conventional X-ray source.
引用
收藏
页码:1193 / 1194
页数:2
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