Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea

被引：29
|
作者：
Skov, LK
Mirza, O
Henriksen, A
De Montalk, GP
Remaud-Simeon, M
Sarcabal, P
Willemot, RM
Monsan, P
Gajhede, M
机构：
[1] Univ Copenhagen, Dept Chem, Prot Struct Grp, DK-2100 Copenhagen, Denmark
[2] Inst Natl Sci Appl, F-31077 Toulouse 4, France
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2000年 / 56卷
关键词：
D O I：
10.1107/S0907444999015887
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Recombinant amylosucrase from Neisseria polysaccharea was crystallized by the vapour-diffusion procedure in the presence of polyethylene glycol 6000. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 95.7, b = 117.2, c = 62.1 Angstrom, and diffract to 1.6 Angstrom resolution. A p-chloromercuribenzene sulfonate (pcmbs) derivative has been identified and a selenomethionine-substituted protein has been produced and crystallized.
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收藏
页码：203 / 205
页数：3
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