Inhibition of CRISPR-Cas9 with Bacteriophage Proteins

被引:335
|
作者
Rauch, Benjamin J. [1 ,2 ]
Silvis, Melanie R. [1 ,3 ]
Hultquist, Judd F. [2 ,4 ,5 ]
Waters, Christopher S. [1 ,2 ,3 ]
McGregor, Michael J. [2 ,4 ,5 ]
Krogan, Nevan J. [2 ,4 ,5 ]
Bondy-Denomy, Joseph [1 ,2 ]
机构
[1] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94158 USA
[2] Univ Calif San Francisco, Quantitat Biosci Inst, QBI, San Francisco, CA 94158 USA
[3] Univ Calif San Francisco, Tetrad Grad Program, San Francisco, CA 94158 USA
[4] Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94158 USA
[5] J David Gladstone Inst, San Francisco, CA 94158 USA
基金
美国国家科学基金会;
关键词
LISTERIA-MONOCYTOGENES; RNA; SEQUENCE; SYSTEMS; DNA; IDENTIFICATION; TRANSCRIPTION; ENDONUCLEASE; INTEGRATION; EXPRESSION;
D O I
10.1016/j.cell.2016.12.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacterial CRISPR-Cas systems utilize sequencespecific RNA-guided nucleases to defend against bacteriophage infection. As a countermeasure, numerous phages are known that produce proteins to block the function of class 1 CRISPR-Cas systems. However, currently no proteins are known to inhibit the widely used class 2 CRISPR-Cas9 system. To find these inhibitors, we searched cas9-containing bacterial genomes for the co-existence of a CRISPR spacer and its target, a potential indicator for CRISPR inhibition. This analysis led to the discovery of four unique type II-A CRISPR-Cas9 inhibitor proteins encoded by Listeria monocytogenes prophages. More than half of L. monocytogenes strains with cas9 contain at least one prophage-encoded inhibitor, suggesting widespread CRISPR-Cas9 inactivation. Two of these inhibitors also blocked the widely used Streptococcus pyogenes Cas9 when assayed in Escherichia coli and human cells. These natural Cas9-specific "anti-CRISPRs'' present tools that can be used to regulate the genome engineering activities of CRISPR-Cas9.
引用
收藏
页码:150 / +
页数:19
相关论文
共 50 条
  • [1] Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides
    Yan-ru Cui
    Shao-jie Wang
    Jun Chen
    Jie Li
    Wenzhang Chen
    Shuyue Wang
    Bing Meng
    Wei Zhu
    Zhuhong Zhang
    Bei Yang
    Biao Jiang
    Guang Yang
    Peixiang Ma
    Jia Liu
    [J]. Genome Biology, 21
  • [2] Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides
    Cui, Yan-ru
    Wang, Shao-jie
    Chen, Jun
    Li, Jie
    Chen, Wenzhang
    Wang, Shuyue
    Meng, Bing
    Zhu, Wei
    Zhang, Zhuhong
    Yang, Bei
    Jiang, Biao
    Yang, Guang
    Ma, Peixiang
    Liu, Jia
    [J]. GENOME BIOLOGY, 2020, 21 (01)
  • [3] CRISPR-Cas9 Based Bacteriophage Genome Editing
    Zhang, Xueli
    Zhang, Chaohui
    Liang, Caijiao
    Li, Bizhou
    Meng, Fanmei
    Ai, Yuncan
    [J]. MICROBIOLOGY SPECTRUM, 2022, 10 (04):
  • [4] Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type II Anti-CRISPR Proteins
    Hwang, Sungwon
    Maxwell, Karen L.
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2023, 435 (07)
  • [5] Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins
    Zhu, Yalan
    Gao, Ang
    Zhan, Qi
    Wang, Yong
    Feng, Han
    Liu, Songqing
    Gao, Guangxia
    Serganov, Alexander
    Gao, Pu
    [J]. MOLECULAR CELL, 2019, 74 (02) : 296 - +
  • [6] Fluorescent labeling of bacteriophage T7 by CRISPR-Cas9
    Znobishcheva, E. A.
    Morozova, N. E.
    Khodorkovskii, M. A.
    [J]. INTERNATIONAL CONFERENCE PHYSICA.SPB/2019, 2019, 1400
  • [7] Engineered Anti-CRISPR Proteins for Precision Control of CRISPR-Cas9
    Bubeck, Felix
    Hoffmann, Mareike D.
    Harteveld, Zander
    Aschenbrenner, Sabine
    Bietz, Andreas
    Notbohm, Judith
    Stengl, Christina
    Mathony, Jan
    Waldhauer, Max C.
    Dietz, Laura
    Boerner, Kathleen
    Fakhiri, Julia
    Schmelas, Carolin
    Grimm, Dirk
    Correia, Bruno E.
    Eils, Roland
    Niopek, Dominik
    [J]. MOLECULAR THERAPY, 2019, 27 (04) : 297 - 297
  • [8] Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9
    Bubeck, Felix
    Hoffmann, Mareike D.
    Harteveld, Zander
    Aschenbrenner, Sabine
    Bietz, Andreas
    Waldhauer, Max C.
    Boerner, Kathleen
    Fakhiri, Julia
    Schmelas, Carolin
    Dietz, Laura
    Grimm, Dirk
    Correia, Bruno E.
    Eils, Roland
    Niopek, Dominik
    [J]. NATURE METHODS, 2018, 15 (11) : 924 - +
  • [9] Temperature-Responsive Competitive Inhibition of CRISPR-Cas9
    Jiang, Fuguo
    Liu, Jun-Jie
    Osuna, Beatriz A.
    Xu, Michael
    Berry, Joel D.
    Rauch, Benjamin J.
    Nogales, Eva
    Bondy-Denomy, Joseph
    Doudna, Jennifer A.
    [J]. MOLECULAR CELL, 2019, 73 (03) : 601 - +
  • [10] Efficient Genome Engineering of a Virulent Klebsiella Bacteriophage Using CRISPR-Cas9
    Shen, Juntao
    Zhou, Jinjie
    Chen, Guo-Qiang
    Xiu, Zhi-Long
    [J]. JOURNAL OF VIROLOGY, 2018, 92 (17)