Genetically Encoded Biosensors Based on Fluorescent Proteins

被引:29
|
作者
Kim, Hyunbin [1 ,2 ]
Ju, Jeongmin [1 ,2 ]
Lee, Hae Nim [1 ,3 ]
Chun, Hyeyeon [1 ]
Seong, Jihye [1 ,2 ,3 ]
机构
[1] Korea Inst Sci & Technol KIST, Brain Sci Inst, Seoul 02792, South Korea
[2] Korea Univ Sci & Technol, KIST Sch, Div Biomed Sci & Technol, Seoul 02792, South Korea
[3] Kyung Hee Univ, Dept Converging Sci & Technol, Seoul 02453, South Korea
关键词
fluorescent protein; genetically encoded biosensor; FRET; ddFP; BiFC; split FP; circular permutation; fluorescent timer; LIVING CELLS; IN-VIVO; COMPLEMENTATION SYSTEM; TERNARY COMPLEXES; AUTOPHAGIC FLUX; GREEN; RED; FRET; VISUALIZATION; DYNAMICS;
D O I
10.3390/s21030795
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs.
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页码:1 / 18
页数:18
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