Genetically Encoded Biosensors Based on Fluorescent Proteins

被引:29
|
作者
Kim, Hyunbin [1 ,2 ]
Ju, Jeongmin [1 ,2 ]
Lee, Hae Nim [1 ,3 ]
Chun, Hyeyeon [1 ]
Seong, Jihye [1 ,2 ,3 ]
机构
[1] Korea Inst Sci & Technol KIST, Brain Sci Inst, Seoul 02792, South Korea
[2] Korea Univ Sci & Technol, KIST Sch, Div Biomed Sci & Technol, Seoul 02792, South Korea
[3] Kyung Hee Univ, Dept Converging Sci & Technol, Seoul 02453, South Korea
关键词
fluorescent protein; genetically encoded biosensor; FRET; ddFP; BiFC; split FP; circular permutation; fluorescent timer; LIVING CELLS; IN-VIVO; COMPLEMENTATION SYSTEM; TERNARY COMPLEXES; AUTOPHAGIC FLUX; GREEN; RED; FRET; VISUALIZATION; DYNAMICS;
D O I
10.3390/s21030795
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs.
引用
收藏
页码:1 / 18
页数:18
相关论文
共 50 条
  • [21] Functional principles of genetically encoded fluorescent biosensors for metabolism and their quantitative use
    Koveal, Dorothy
    JOURNAL OF NEUROCHEMISTRY, 2024, 168 (05) : 496 - 505
  • [22] Using genetically encoded fluorescent biosensors to interrogate ovarian cancer metabolism
    Shree Bose
    Haipei Yao
    Qiang Huang
    Regina Whitaker
    Christopher D. Kontos
    Rebecca A. Previs
    Xiling Shen
    Journal of Ovarian Research, 15
  • [23] Genetically encoded lipid biosensors
    Wills, Rachel C.
    Goulden, Brady D.
    Hammond, Gerald R. V.
    MOLECULAR BIOLOGY OF THE CELL, 2018, 29 (13) : 1526 - 1532
  • [24] Genetically Encoded Fluorescent Biosensors for Live-Cell Visualization of Protein Phosphorylation
    Oldach, Laurel
    Zhang, Jin
    CHEMISTRY & BIOLOGY, 2014, 21 (02): : 186 - 197
  • [25] Seeing Neurodegeneration in a New Light Using Genetically Encoded Fluorescent Biosensors and iPSCs
    Stellon, David
    Talbot, Jana
    Hewitt, Alex W. W.
    King, Anna E. E.
    Cook, Anthony L. L.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (02)
  • [26] Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors
    Depry, Charlene
    Mehta, Sohum
    Zhang, Jin
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2013, 465 (03): : 373 - 381
  • [27] Development of genetically encoded fluorescent protein FRET- based biosensors for matrix metalloproteinase-2
    Ferreira, A. L. B. J.
    Belal, A.
    Ali, M. A.
    Fan, X.
    Holt, A.
    Campbell, R.
    Schulz, R.
    CARDIOVASCULAR RESEARCH, 2012, 93 : S71 - S71
  • [28] Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors
    Charlene Depry
    Sohum Mehta
    Jin Zhang
    Pflügers Archiv - European Journal of Physiology, 2013, 465 : 373 - 381
  • [29] Flavin-binding fluorescent proteins as genetically encoded photosensitizers
    Gensch, T.
    JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 2018, 50 (06) : 495 - 496
  • [30] An update on genetically encoded lipid biosensors
    Hammond, Gerald R., V
    Ricci, Morgan M. C.
    Weckerly, Claire C.
    Wills, Rachel C.
    MOLECULAR BIOLOGY OF THE CELL, 2022, 33 (05)