A Simple Self-Calibrating Method To Measure the Height of Fluorescent Molecules and Beads at Nanoscale Resolution

被引：3
|
作者：
Kerssemakers, Jacob W. J. [1 ]
Blosser, Timothy R. [1 ]
Dekker, Cees [1 ]
机构：
[1] Delft Univ Technol, Dept Bionanosci, Kavli Inst Nanosci, NL-2628 CJ Delft, Netherlands
来源：
NANO LETTERS | 2014年 / 14卷 / 08期
基金：
欧洲研究理事会;
关键词：
Height determination; fluorescence; total internal reflection fluorescence; nanoscale resolution; INTERFERENCE; LOCALIZATION; MICROSCOPY; TRACKING;
D O I：
10.1021/nl501434v
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We describe a simple self-calibrating technique, incident-beam interference sweeping, for measuring the height of fluorescent labels. Using a tilted back-reflecting mirror and a scanning laser beam, a modulated fluorescence emission allows height determination of a label from a surface with a resolution of similar to 3 nm. In addition, we show that the absolute distance of a label from the top-mounted mirror can be determined with a resolution of a few tens of nanometers over a micrometer range.
引用
收藏
页码：4469 / 4475
页数：7
相关论文
共 50 条
  • [11] A Self-Calibrating sub-picosecond resolution Digital-to-Time Converter
    Nagaraj, Geetha
    Miller, Scott
    Stengel, Bob
    Cafaro, Gio
    Gradishar, Tom
    Olson, Scott
    Hekmann, Ralf
    2007 IEEE/MTT-S INTERNATIONAL MICROWAVE SYMPOSIUM DIGEST, VOLS 1-6, 2007, : 2192 - +
  • [12] How To Characterize Individual Nanosize Liposomes with Simple Self-Calibrating Fluorescence Microscopy
    Mortensen, Kim, I
    Tassone, Chiara
    Ehrlich, Nicky
    Andresen, Thomas L.
    Flyvbjerg, Henrik
    NANO LETTERS, 2018, 18 (05) : 2844 - 2851
  • [13] A Self-Calibrating Method for Photogeometric Acquisition of 3D Objects
    Aliaga, Daniel G.
    Xu, Yi
    IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE, 2010, 32 (04) : 747 - 754
  • [14] Self-Calibrating Mechanochromic Fluorescent Polymers Based on Encapsulated Excimer-Forming Dyes
    Calvino, Celine
    Guha, Anirvan
    Weder, Christoph
    Schrettl, Stephen
    ADVANCED MATERIALS, 2018, 30 (19)
  • [15] SELF-CALIBRATING METHOD FOR ESTIMATING SOLAR RADIATION FROM AIR TEMPERATURE
    Allen, Richard G.
    JOURNAL OF HYDROLOGIC ENGINEERING, 1997, 2 (02) : 56 - 67
  • [16] AN IMPROVED SELF-CALIBRATING METHOD FOR PARAMETER EXTRACTION FROM IMPATT DIODES
    GOELLER, T
    BOCKMAIR, U
    LUY, JF
    HAUSNER, J
    RUSSER, P
    AEU-ARCHIV FUR ELEKTRONIK UND UBERTRAGUNGSTECHNIK-INTERNATIONAL JOURNAL OF ELECTRONICS AND COMMUNICATIONS, 1992, 46 (02): : 107 - 110
  • [17] Self-Calibrating Gain Stabilization Method for Applications Using Silicon Photomultipliers
    Baszczyk, Mateusz
    Dorosz, Piotr
    Glab, Sebastian
    Kucewicz, Wojciech
    Mik, Lukasz
    Sapor, Maria
    2013 IEEE NUCLEAR SCIENCE SYMPOSIUM AND MEDICAL IMAGING CONFERENCE (NSS/MIC), 2013,
  • [18] USE OF SELF-CALIBRATING CATALYTIC PROBES TO MEASURE FREE-STREAM ATOM CONCENTRATION
    REDDY, NM
    PHYSICS OF FLUIDS, 1967, 10 (9P1) : 1905 - &
  • [19] Self-Calibrating Bipartite Fluorescent Sensor for Nitroreductase Activity and Its Application to Cancer and Hypoxic Cells
    Yoon, Shin A.
    Chun, Jieun
    Kang, Chulhun
    Lee, Min Hee
    ACS APPLIED BIO MATERIALS, 2021, 4 (03) : 2052 - 2057
  • [20] Self-Calibrating Method for Motor Performance Data Without Dynamometer: A Soft Dyno
    Smiley, R. Gene
    2019 IEEE TRANSPORTATION ELECTRIFICATION CONFERENCE AND EXPO (ITEC), 2019,
12345