Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy

被引:28
|
作者
Mlynarikova, Katarina [1 ,2 ]
Samek, Ota [3 ]
Bernatova, Silvie [3 ]
Ruzicka, Filip [1 ,2 ]
Jezek, Jan [3 ]
Haronikova, Andrea [4 ]
Siler, Martin [3 ]
Zemanek, Pavel [3 ]
Hola, Veronika [1 ,2 ]
机构
[1] Fac Med, Dept Microbiol, Brno 65691, Czech Republic
[2] St Annes Fac Hosp, Brno 65691, Czech Republic
[3] Acad Sci Czech Republ, Inst Sci Instruments, Vvi, CS-61264 Brno, Czech Republic
[4] Brno Univ Technol, Ctr Mat Res, Brno 61200, Czech Republic
关键词
Raman spectroscopy; bacteria; yeasts; culture media; COAGULASE-NEGATIVE STAPHYLOCOCCI; SINGLE-CELL ANALYSIS; CANDIDA-ALBICANS; BIOFILM FORMATION; RAPID IDENTIFICATION; FUNGAL PATHOGEN; EPIDERMIDIS; INFECTIONS; MICROSPECTROSCOPY; BACTERIAL;
D O I
10.3390/s151129635
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans). Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar) and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.
引用
收藏
页码:29635 / 29647
页数:13
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