Rapid analysis of multiple parameters of CHO cell culture media using Raman spectroscopy

被引：0
|
作者：
Yan X. [1 ]
Shen L.-J. [1 ]
Xu W.-Y. [2 ]
Pan H.-H. [2 ]
Nie L. [1 ]
Wang H.-B. [2 ]
Li W.-L. [1 ]
Qu H.-B. [1 ]
机构：
[1] Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou
[2] Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou
来源：
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | 2019年 / 33卷 / 04期
关键词：
CHO cell culture; Monoclonal antibody; Partial least squares; Raman spectroscopy; Rapid analysis;
D O I：
10.3969/j.issn.1003-9015.2019.04.013
中图分类号：
学科分类号：
摘要：
In order to rapidly analyze Chinese hamster ovary (CHO) cell culture media, samples were collected during bioreactor runs and reference values of glucose concentration, lactate concentration, glutamate concentration, glutamine concentration, viable cell density, total cell density, cell viability and monoclonal antibody titer were obtained off-line. Raman spectra of cell culture supernatants were collected and partial least squares (PLS) was used to develop multivariate calibration models between Raman spectra and parameters. Reasonable principal component numbers, high coefficient of determination (R2), low root mean square error of calibration (RMSEC), low root mean square error of prediction (RMSEP), and slight differences between RMSEC and RMSEP were observed in each model, which demonstrates satisfactory predictive capacity of the developed models. The results show that the proposed method is expected to be used in off-line and rapid determination of multiple parameters in CHO cell culture processes, which can provide guidelines for in-line real-time monitoring and feedback control. © 2019, Editorial Board of "Journal of Chemical Engineering of Chinese Universities". All right reserved.
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页码：872 / 877
页数：5
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