Embryo viability indexing using Raman spectroscopy of spent culture media

被引：5
|
作者：
Parlatan, Ugur [1 ,2 ]
Basar, Gunay [1 ]
Bavili, Nima [1 ]
Yumru, Harika [3 ]
Sag, Fatih [3 ]
Bulgurcuoglu, Sibel [3 ]
Bastu, Ercan [3 ]
机构：
[1] Istanbul Tech Univ, Dept Engn Phys, Fac Sci & Letters, TR-34469 Istanbul, Turkey
[2] Istanbul Univ, Dept Engn Sci, Fac Engn, Istanbul, Turkey
[3] Istanbul Univ, Dept Obstet & Gynecol, Sch Med, Istanbul, Turkey
来源：
SPECTROSCOPY LETTERS | 2016年 / 49卷 / 07期
关键词：
Band component analysis; embryo viability; in vitro fertilization; Raman spectroscopy; IN-VITRO FERTILIZATION; AMINO-ACID TURNOVER; INFRARED-SPECTROSCOPY; MICROSCOPY; PREDICTION; CELLS; WOMEN; VIVO; IVF;
D O I：
10.1080/00387010.2016.1198814
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm 1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.
引用
收藏
页码：458 / 463
页数：6
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