Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP

被引：389

作者：

Fanello, C

Santolamazza, F

della Torre, A

机构：

[1] Univ Roma La Sapienza, Dipartimento Sci Sanita Publ, Sez Parassitol, I-00185 Rome, Italy

[2] London Sch Hyg & Trop Med, London WC1, England

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 2002年 / 16卷 / 04期

关键词：

Anopheles gambiae complex; malaria vectors; molecular markers; mosquito identification; PCR-RFLP; sibling species; Africa;

D O I：

10.1046/j.1365-2915.2002.00393.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto , we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.

引用

页码：461 / 464

页数：4

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