Differentiation of sturgeon species by PCR-RFLP

被引：39
|
作者：
Wolf, C [1 ]
Hübner, P [1 ]
Lüthy, J [1 ]
机构：
[1] Univ Bern, Dept Chem & Biochem, Food Chem Lab, CH-3012 Bern, Switzerland
来源：
FOOD RESEARCH INTERNATIONAL | 1999年 / 32卷 / 10期
关键词：
species identification; sturgeon; Acipenser; caviar; PCR; RFLP; cyt b;
D O I：
10.1016/S0963-9969(99)00150-7
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNA(Glu)/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia. (C) 2000 Canadian Institute of Food Science and Technology. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：699 / 705
页数：7
相关论文
共 50 条
  • [1] Differentiation of Xanthomonas species Pathogenic to Sugarcane by PCR-RFLP Analysis
    S.A.L. Destéfano
    I.M.G. Almeida
    J. Rodrigues Neto
    M. Ferreira
    D.M. Balani
    European Journal of Plant Pathology, 2003, 109 : 283 - 288
  • [2] Differentiation of Xanthomonas species pathogenic to sugarcane by PCR-RFLP analysis
    Destéfano, SAL
    Almeida, IMG
    Neto, JR
    Ferreira, M
    Balani, DM
    EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2003, 109 (03) : 283 - 288
  • [3] A PCR-RFLP method for the simultaneous differentiation of three Entamoeba species
    Fontecha, Gustavo A.
    Garcia, Kimberly
    Mercedes Rueda, Maria
    Sosa-Ochoa, Wilfredo
    Lourdes Sanchez, Ana
    Leiva, Byron
    EXPERIMENTAL PARASITOLOGY, 2015, 151 : 80 - 83
  • [4] PCR-RFLP analysis of mitochondrial DNA:: Differentiation of fish species
    Wolf, C
    Burgener, M
    Hübner, P
    Lüthy, J
    LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY, 2000, 33 (02): : 144 - 150
  • [5] Differentiation of Xanthomonas species by PCR-RFLP of rpfB and atpD genes
    Simoes, Tiago H. N.
    Goncalves, Edmilson R.
    Rosato, Yoko B.
    Mehta, Angela
    FEMS MICROBIOLOGY LETTERS, 2007, 271 (01) : 33 - 39
  • [6] PCR-RFLP ANALYSIS OF DNA FOR THE DIFFERENTIATION OF FISH SPECIES IN SEAFOOD SAMPLES
    Nebola, Mojmir
    Borilova, Gabriela
    Kasalova, Janka
    BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY, 2010, 54 (01) : 49 - 53
  • [7] A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species
    Mirhendi, H
    Makimura, K
    Zomorodian, K
    Yamada, T
    Sugita, T
    Yamaguchi, H
    JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 61 (02) : 281 - 284
  • [8] Differentiation of cattle species in beef by PCR-RFLP of mitochondrial and satellite DNA
    Verkaar, ELC
    Nijman, IJ
    Boutaga, K
    Lenstra, JA
    MEAT SCIENCE, 2002, 60 (04) : 365 - 369
  • [9] DIFFERENTIATION OF NOCARDIA FROM RAPIDLY GROWING MYCOBACTERIUM SPECIES BY PCR-RFLP ANALYSIS
    LUNGU, O
    LATTA, PD
    WEITZMAN, I
    SILVERSTEIN, S
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1994, 18 (01) : 13 - 18
  • [10] Novel PCR-RFLP system based on rpoB gene for differentiation of Cronobacter species
    Vlach, Jiri
    Javurkova, Barbora
    Karamonova, Ludmila
    Blazkova, Martina
    Fukal, Ladislav
    FOOD MICROBIOLOGY, 2017, 62 : 1 - 8
12345