Differentiation of sturgeon species by PCR-RFLP

被引：39
|
作者：
Wolf, C [1 ]
Hübner, P [1 ]
Lüthy, J [1 ]
机构：
[1] Univ Bern, Dept Chem & Biochem, Food Chem Lab, CH-3012 Bern, Switzerland
来源：
FOOD RESEARCH INTERNATIONAL | 1999年 / 32卷 / 10期
关键词：
species identification; sturgeon; Acipenser; caviar; PCR; RFLP; cyt b;
D O I：
10.1016/S0963-9969(99)00150-7
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNA(Glu)/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia. (C) 2000 Canadian Institute of Food Science and Technology. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：699 / 705
页数：7
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