Development of 27 trinucleotide microsatellite markers for Saccharina japonica using next generation sequencing technology

被引：24

作者：

Zhang, Linan ^{[1
]}

Peng, Jie ^{[1
]}

Li, Xiaojie ^{[1
]}

Liu, Yanling ^{[1
]}

Cui, Cuiju ^{[1
]}

Wu, Hao ^{[2
]}

Wu, Ruina ^{[1
]}

Tian, Pingping ^{[1
]}

Li, Yan ^{[1
]}

机构：

[1] Shandong Oriental Ocean Scitech Co Ltd, Natl Algae Engn & Tech Res Ctr, Yantai 264003, Shandong, Peoples R China

[2] Qingdao Agr Univ, Qingdao 266109, Shandong, Peoples R China

来源：

CONSERVATION GENETICS RESOURCES | 2014年 / 6卷 / 02期

关键词：

Microsatellite; Saccharina japonica; Saccharina longissima; Next generation DNA sequencing; WILD;

D O I：

10.1007/s12686-013-0089-0

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Twenty-seven polymorphic trinucleotide microsatellite DNA markers were developed from Saccharina japonica, through paired-end Illumina sequencing data. Sixty-three gametophyte clones of the species, preserved indoor as germplasm resources were genotyped using these markers. The number of alleles ranged from 2 to 7 with an average 4.7 per locus. The gene diversity and expected heterozygosity varied from 0.171 to 0.774 and from 0.174 to 0.793, respectively. Sixteen loci could be successfully amplified in Saccharina longissima with 12 loci being polymorphic. These novel microsatellite DNA markers will be extremely useful in germplasm resource conservation and management of S. japonica and also in its related species S. longissima.

引用

页码：341 / 344

页数：4

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