DEVELOPMENT OF MICROSATELLITE MARKERS FOR SARGENTODOXA CUNEATA (LARDIZABALACEAE) USING NEXT-GENERATION SEQUENCING TECHNOLOGY

被引：4
|
作者：
Sun, Zhi-Xia [1 ,2 ]
Ye, Lin-Jiang [2 ,3 ]
Zhang, Fei [2 ]
Hu, Wan [1 ,2 ]
Fan, Deng-Mei [2 ]
Zhang, Zhi-Yong [2 ]
机构：
[1] Jiangxi Agr Univ, Coll Forestry, Nanchang 330045, Jiangxi, Peoples R China
[2] Jiangxi Agr Univ, Lab Subtrop Biodivers, Nanchang 330045, Jiangxi, Peoples R China
[3] Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Yunnan, Peoples R China
来源：
APPLICATIONS IN PLANT SCIENCES | 2016年 / 4卷 / 05期
基金：
中国国家自然科学基金;
关键词：
genetic diversity; Illumina MiSeq; Lardizabalaceae; microsatellite markers; Sargentodoxa cuneata; SOFTWARE;
D O I：
10.3732/apps.1600003
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: Microsatellite loci were developed for a woody deciduous liana, Sargentodoxa cuneata (Lardizabalaceae), to help infer the evolutionary histories of ancient monotypic genera in subtropical China. Methods and Results: Using next-generation sequencing (Illumina MiSeq) technology, 21 polymorphic primer sets were identified in three wild populations. The number of alleles per locus ranged from one to seven. The expected and observed heterozygosities varied from 0 to 0.788 and 0 to 0.917, respectively. Transferability analyses were performed in Stauntonia chinensis, Akebia trifoliata, and A. quinata. Eighteen (85.7%), 18 (85.7%), and 17 (81.0%) markers were successfully amplified, respectively. Conclusions: The newly developed markers will facilitate further studies on genetic diversity and phylogeographic patterns throughout the distributional range of S. cuneata. This set of microsatellite primers represents the second report on molecular markers in Lardizabalaceae.
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页数：4
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