Development of microsatellite markers for Suriana maritima (Surianaceae) using next-generation sequencing technology

被引:3
|
作者
Chen, W. S. [1 ,2 ]
Zhao, G. [2 ]
Jian, S. G. [1 ]
Wang, Z. F. [1 ]
机构
[1] Chinese Acad Sci, South China Bot Garden, Guangzhou, Guangdong, Peoples R China
[2] Zhongkai Univ Agr & Engn, Guangzhou, Guangdong, Peoples R China
来源
GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 04期
关键词
Suriana maritima; Surianaceae; Microsatellite markers; Next-generation sequencing; Paracel islands; STACKS;
D O I
10.4238/2015.October.29.31
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Our objective was to develop microsatellite markers for use in assessing genetic variation in the small shrub or tree species Suriana maritima (Surianaceae). In China, this species is found only as a few fragmented populations and individuals on the Paracel Islands. Using next-generation genome sequencing methodology, we developed 17 novel microsatellite markers for S. maritima. Fifty-four individuals from six populations of S. maritima were examined for polymorphisms; only one allele was detected for each of the markers. Microsatellite loci developed indicate a complete absence of genetic diversity for S. maritima on the Paracel Islands in China. These markers will be useful for examining genetic variation among S. maritima populations in other areas of the world.
引用
收藏
页码:14115 / 14118
页数:4
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