Biochemical and functional characterization of the cis-spliceosomal U1 small nuclear RNP from Trypanosoma brucei

被引：20
|
作者：
Palfi, Z
Lane, WS
Bindereif, A
机构：
[1] Univ Giessen, Inst Biochem, D-35392 Giessen, Germany
[2] Harvard Univ, Harvard Microchem & Proteom Anal Facil, Cambridge, MA 02138 USA
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2002年 / 121卷 / 02期
关键词：
small nuclear RNA; snRNA; snRNP; splicing; trypanosomes;
D O I：
10.1016/S0166-6851(02)00044-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent studies in the trypanosome system have revealed that in addition to trans splicing of a short spliced leader (SL) exon, there is also cis splicing of internal introns. It has been suggested that cis splicing requires base-pairing of U1 small nuclear RNA (snRNA) and the 5' splice site. We have cloned the gene for U I snRNA from Trypanosoma brucei and characterized the U I snRNP. Based on immunoprecipitation and direct mass-spectrometric protein analysis the U1 snRNP contains the common Sm core found also in the known trans-spliceosomal snRNPs U2, U4/U6, and U5. The 5' end of U I snRNA in the U I snRNP is accessible for and functional in specific recognition of the 5' splice site of the poly(A) polymerase intron. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码：233 / 243
页数：11
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