Biochemical and functional characterization of the cis-spliceosomal U1 small nuclear RNP from Trypanosoma brucei

Recent studies in the trypanosome system have revealed that in addition to trans splicing of a short spliced leader (SL) exon, there is also cis splicing of internal introns. It has been suggested that cis splicing requires base-pairing of U1 small nuclear RNA (snRNA) and the 5' splice site. We have cloned the gene for U I snRNA from Trypanosoma brucei and characterized the U I snRNP. Based on immunoprecipitation and direct mass-spectrometric protein analysis the U1 snRNP contains the common Sm core found also in the known trans-spliceosomal snRNPs U2, U4/U6, and U5. The 5' end of U I snRNA in the U I snRNP is accessible for and functional in specific recognition of the 5' splice site of the poly(A) polymerase intron. (C) 2002 Elsevier Science B.V. All rights reserved.