A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

被引：21
|
作者：
Peng, Xiayu [1 ]
Kang, Lichao [1 ,2 ]
Pang, Fangqin [2 ]
Li, Hongmin [2 ]
Luo, Ruifeng [2 ]
Luo, Xiaoling [1 ,2 ]
Sun, Fengxia [1 ,2 ]
机构：
[1] State Key Lab Sheep Genet Improvement & Hlth Bree, Shihezi 832000, Peoples R China
[2] Xinjiang Acad Agr & Reclamat Sci, Anal & Testing Ctr, Shihezi 832000, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2018年 / 29卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Lateral flow strip; signal amplification; BPA; food safety; CHROMATOGRAPHY-MASS SPECTROMETRY; LABEL-FREE; POLYAMIDOAMINE DENDRIMER; AQUEOUS SAMPLES; PAMAM DENDRIMER; WATER SAMPLES; NANOPARTICLES; EXPOSURE; MICROEXTRACTION; IMMUNOASSAY;
D O I：
10.1080/09540105.2017.1365822
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A signal-enhanced lateral flow strip biosensor has been constructed for ultrasensitive and on-site visual detection of bisphenol A (BPA). The signal amplification principle is based on the capacity of binding a large number of antibody-assembled gold nanoparticle (AuNP-antibody) conjugates of the poly amidoamine (PAMAM), which bears a multitude of amino groups. This signal-enhanced method could achieve BPA detection at 10ppb based on naked eye visual observation, which represents an at least 50-fold improvement of the sensitivity of a traditional method without PAMAM. Moreover, good linearity was observed in the range from 2 to 100ppb after quantitative analysis using the ImageJ software. This signal amplification method could be adopted as a potential generous technique in different fields.
引用
收藏
页码：216 / 227
页数：12
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