Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection

被引：7

作者：

Reverte, Maeva ^{[1
]}

Vasseur, Jean-Jacques ^{[1
]}

Smietana, Michael ^{[1
]}

机构：

[1] Univ Montpellier, CNRS, ENSCM, Inst Biomol Max Mousseron,UMR 5247, F-34095 Montpellier, France

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2015年 / 13卷 / 43期

关键词：

NUCLEOTIDE POLYMORPHISM DETECTION; SYBR GREEN I; DNA DETECTION; SIGNAL AMPLIFICATION; CHEMICAL-MODIFICATION; MOLECULAR BEACONS; GENETIC-ANALYSIS; EXONUCLEASE III; HYBEACON PROBES; OLIGONUCLEOTIDES;

D O I：

10.1039/c5ob01815c

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

5'-End boronic acid-modified oligonucleotides were evaluated against various nucleases at single and double stranded levels. The results show that these modifications induce a high resistance to degradation by calf-spleen and snake venom phosphodiesterases. More importantly, this eventually led to the development of a new label-free enzyme-assisted fluorescence-based method for single mismatch detection.

引用

页码：10604 / 10608

页数：5

共 50 条

[1] Label-free visual detection of nucleic acids in biological samples with single-base mismatch detection capability
Song, Yanling
Zhang, Weiting
An, Yuan
Cui, Liang
Yu, Chundong
Zhu, Zhi
Yang, Chaoyong James
CHEMICAL COMMUNICATIONS, 2012, 48 (04) : 576 - 578
[2] Biomimetic nanochannels based biosensor for ultrasensitive and label-free detection of nucleic acids
Sun, Zhongyue
Liao, Tangbin
Zhang, Yulin
Shu, Jing
Zhang, Hong
Zhang, Guo-Jun
BIOSENSORS & BIOELECTRONICS, 2016, 86 : 194 - 201
[3] Label-Free Nuclease Assay with Long-Term Stability
Liu, Rui
Hu, Jianyu
Chen, Yongxin
Jiang, Min
Lv, Yi
ANALYTICAL CHEMISTRY, 2019, 91 (13) : 8691 - 8696
[4] Label-free nucleic acids detection based on DNA templated silver nanoclusters fluorescent probe
Zhao, Haiyan
Wang, Lei
Zhu, Jing
Wei, Haiping
Jiang, Wei
TALANTA, 2015, 138 : 163 - 168
[5] Label-free quantitative detection of nucleic acids based on surface-immobilized DNA intercalators
Lee, Hun Joo
Lee, Soo Suk
SENSORS AND ACTUATORS B-CHEMICAL, 2017, 241 : 1310 - 1315
[6] Colorimetric Sensing of α-Amino Acids and Its Application for the "Label-Free" Detection of Protease
Lou, Xiaoding
Zhang, Liyao
Qin, Jingui
Li, Zhen
LANGMUIR, 2010, 26 (03) : 1566 - 1569
[7] ANYL 136-Label- and label-free signal amplification pathways for sensitive electroanalytical detection of trace nucleic acids
Kawde, Abdel-Nasser M.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2008, 236
[8] Boron nitride nanosheet modified label-free electrochemical immunosensor for cancer antigen 125 detection
Ondes, Baha
Evli, Sinem
Uygun, Murat
Uygun, Deniz Aktas
BIOSENSORS & BIOELECTRONICS, 2021, 191
[9] Label-Free Detection of Lectins on Carbohydrate-Modified Boron-Doped Diamond Surfaces
Szunerits, Sabine
Niedziolka-Joensson, Joanna
Boukherroub, Rabah
Woisel, Patrice
Baumann, Jean-Sebastien
Siriwardena, Aloysius
ANALYTICAL CHEMISTRY, 2010, 82 (19) : 8203 - 8210
[10] Integrated microelectronic device for label-free nucleic acid amplification and detection
Hou, Chih-Sheng Johnson
Godin, Michel
Payer, Kristofor
Chakrabarti, Raj
Manalis, Scott R.
LAB ON A CHIP, 2007, 7 (03) : 347 - 354

← 1 2 3 4 5 →