Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection

被引：7
|
作者：
Reverte, Maeva [1 ]
Vasseur, Jean-Jacques [1 ]
Smietana, Michael [1 ]
机构：
[1] Univ Montpellier, CNRS, ENSCM, Inst Biomol Max Mousseron,UMR 5247, F-34095 Montpellier, France
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2015年 / 13卷 / 43期
关键词：
NUCLEOTIDE POLYMORPHISM DETECTION; SYBR GREEN I; DNA DETECTION; SIGNAL AMPLIFICATION; CHEMICAL-MODIFICATION; MOLECULAR BEACONS; GENETIC-ANALYSIS; EXONUCLEASE III; HYBEACON PROBES; OLIGONUCLEOTIDES;
D O I：
10.1039/c5ob01815c
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
5'-End boronic acid-modified oligonucleotides were evaluated against various nucleases at single and double stranded levels. The results show that these modifications induce a high resistance to degradation by calf-spleen and snake venom phosphodiesterases. More importantly, this eventually led to the development of a new label-free enzyme-assisted fluorescence-based method for single mismatch detection.
引用
收藏
页码：10604 / 10608
页数：5
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