Genome detection of dermatophytes Current findings from the external quality assessment test

被引:2
|
作者
Kupsch, C. [1 ]
Graeser, Y. [1 ]
机构
[1] Charite Univ Med Berlin, Inst Mikrobiol & Infekt Immunol, Hindenburgdamm 27, D-12203 Berlin, Germany
来源
HAUTARZT | 2019年 / 70卷 / 08期
关键词
Molecular diagnostics; Polymerase chain reaction; Reagent kits; diagnostic; Trichophyton rubrum; Species diagnosis; POLYMERASE-CHAIN-REACTION; CAUSATIVE FUNGAL AGENTS; REAL-TIME PCR; DIFFERENTIAL-DIAGNOSIS; RAPID DETECTION; IDENTIFICATION; ONYCHOMYCOSIS; ASSAY;
D O I
10.1007/s00105-019-4435-3
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
In addition to morphological diagnostics, molecular methods have become available for the diagnosis of suspected dermatophytoses. Since March 2016, INSTAND e.V., in cooperation with the National Reference Laboratory for Dermatophytes, has provided an external quality assessment (EQA) test for the genome detection of dermatophytes twice a year. More than half of the participants used commercial kits for the analysis of the samples. All kits with a high level of accuracy correctly determined the presence of Trichophyton rubrum or no dermatophytes. In species diagnostics beyond Trichophyton rubrum there are large differences between the kits. These are examined in more detail based on clinical studies and the results of the EQA test.
引用
收藏
页码:627 / 637
页数:11
相关论文
共 50 条
  • [31] SYPHILIS RAPID TEST EXTERNAL QUALITY ASSESSMENT: BRAZIL'S EXPERIENCE
    Gaspar, Pamela Cristina
    Bigolin, Alisson
    Machado, Hanalydia De Melo
    Pires, Ana Flavia
    Franchini, Miriam
    Benzaken, Adele Schwartz
    Bazzo, Maria Luiza
    SEXUALLY TRANSMITTED INFECTIONS, 2017, 93 : A253 - A254
  • [32] FINDINGS OF AN EXTERNAL QUALITY ASSESSMENT SCHEME FOR DETERMINING ALUMINUM IN DIALYSIS FLUIDS AND WATER
    TAYLOR, A
    BRIGGS, RJ
    CEVIK, C
    CLINICAL CHEMISTRY, 1994, 40 (08) : 1517 - 1521
  • [33] Proficiency Testing/External Quality Assessment: Current Challenges and Future Directions
    Miller, W. Greg
    Jones, Graham R. D.
    Horowitz, Gary L.
    Weykamp, Cas
    CLINICAL CHEMISTRY, 2011, 57 (12) : 1670 - 1680
  • [34] External quality assessments for SARS-CoV-2 genome detection in Austria
    Buchta, Christoph
    Aberle, Stephan W.
    Goerzer, Irene
    Griesmacher, Andrea
    Mueller, Mathias M.
    Neuwirth, Erich
    Puchhammer-Stoeckl, Elisabeth
    Weseslindtner, Lukas
    Camp, Jeremy V.
    WIENER KLINISCHE WOCHENSCHRIFT, 2024, 136 (15-16) : 429 - 438
  • [35] The alcohol biomarker phosphatidylethanol (PEth) - test performance and experiences from routine analysis and external quality assessment
    Helander, Anders
    Hansson, Therese
    SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2023, 83 (06): : 424 - 431
  • [37] External quality assessment (EQA) and alternative assessment procedures (AAPs) in molecular diagnostics: findings of an international survey
    Payne, Deborah A.
    Russomando, Graciela
    Linder, Mark W.
    Baluchova, Katarina
    Ashavaid, Tester
    Steimer, Werner
    Ahmad-Nejad, Parviz
    CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2021, 59 (02) : 301 - 306
  • [38] International External Quality Assessment Study for Molecular Detection of Lassa Virus
    Nikisins, Sergejs
    Rieger, Toni
    Patel, Pranav
    Mueller, Rolf
    Guenther, Stephan
    Niedrig, Matthias
    PLOS NEGLECTED TROPICAL DISEASES, 2015, 9 (05):
  • [39] External quality assessment of cytomegalovirus DNA detection on dried blood spots
    Barbi, Maria
    MacKay, William G.
    Binda, Sandro
    Van Loon, Anton M.
    BMC MICROBIOLOGY, 2008, 8 (1)
  • [40] An external quality assessment scheme for prenatal detection of rare chromosomal abnormalities
    Weng, Binghuan
    Li, Xiao
    CLINICA CHIMICA ACTA, 2012, 413 (21-22) : 1721 - 1724