Genome detection of dermatophytes Current findings from the external quality assessment test

被引:2
|
作者
Kupsch, C. [1 ]
Graeser, Y. [1 ]
机构
[1] Charite Univ Med Berlin, Inst Mikrobiol & Infekt Immunol, Hindenburgdamm 27, D-12203 Berlin, Germany
来源
HAUTARZT | 2019年 / 70卷 / 08期
关键词
Molecular diagnostics; Polymerase chain reaction; Reagent kits; diagnostic; Trichophyton rubrum; Species diagnosis; POLYMERASE-CHAIN-REACTION; CAUSATIVE FUNGAL AGENTS; REAL-TIME PCR; DIFFERENTIAL-DIAGNOSIS; RAPID DETECTION; IDENTIFICATION; ONYCHOMYCOSIS; ASSAY;
D O I
10.1007/s00105-019-4435-3
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
In addition to morphological diagnostics, molecular methods have become available for the diagnosis of suspected dermatophytoses. Since March 2016, INSTAND e.V., in cooperation with the National Reference Laboratory for Dermatophytes, has provided an external quality assessment (EQA) test for the genome detection of dermatophytes twice a year. More than half of the participants used commercial kits for the analysis of the samples. All kits with a high level of accuracy correctly determined the presence of Trichophyton rubrum or no dermatophytes. In species diagnostics beyond Trichophyton rubrum there are large differences between the kits. These are examined in more detail based on clinical studies and the results of the EQA test.
引用
收藏
页码:627 / 637
页数:11
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