Isolation, purification and preliminary characterization of cryophilic proteases of marine origin

被引：10
|
作者：
Salamanca, MH [1 ]
Barría, C [1 ]
Asenjo, JA [1 ]
Andrews, BA [1 ]
机构：
[1] Univ Chile, Dept Chem Engn, Ctr Biochem Engn & Biotechnol, Santiago, Chile
来源：
BIOSEPARATION | 2001年 / 10卷 / 4-5期
关键词：
trypsin-like protease; cryophilic; krill;
D O I：
10.1023/A:1016383212244
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The isolation and preliminary characterization of a trypsin-like protease with high activity at 20 degreesC is described. This protease was isolated from Antarctic krill (Euphasia superba) by a two-step chromatography process and the use of zymogram analyses. The protease has a molecular weight of 30 kDa and a pI of 4.1. Its specific activity at 20 degreesC on BAPNA is 0.5 U/mg.
引用
收藏
页码：237 / 241
页数：5
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